Introduction
Liver cancer is the second leading cause of cancer death worldwide, particularly in men . Hepatocellular carcinoma (HCC) is the most common type (70%–90%) of liver cancer . The multi-targeted tyrosine kinase inhibitor (TKI) sorafenib, with activity against RAF kinase, vascular endothelial growth factor receptor (VEGFR)1–3, platelet-derived growth factor receptor (PDGFR) α and β, FLT3, RET, and KIT , was approved in 2007 and has been used as a first-line systemic therapy for unresectable HCC (uHCC) . However, the clinical benefit of sorafenib is modest and the five-year relative survival of patients with uHCC remains low . Although several TKIs have been tested versus sorafenib in clinical studies of uHCC, all the clinical trials were unsuccessful.
Lenvatinib is an orally administered, multi-targeted TKI that selectively inhibits VEGFR1–3, fibroblast growth factor receptor (FGFR) 1–4, PDGFR α, RET, and KIT . Preclinical studies have revealed that lenvatinib potently blocks VEGF- and FGF-driven angiogenesis, KIT-dependent angiogenesis, RET-fusion/RET mutant tumorigenesis, and VEGFR3-associated lymphangiogenesis . Lenvatinib has been used to treat progressive, locally recurrent or metastatic, radioactive iodine-refractory differentiated thyroid cancer (USA and EU), and unresectable thyroid cancer (Japan) . In addition, lenvatinib in combination with everolimus is approved for the treatment of metastatic renal cell carcinoma following VEGF-targeted therapy . In a recent phase 3 clinical trial of lenvatinib for patients with uHCC (NCT01761266), lenvatinib demonstrated a treatment effect on overall survival by statistical confirmation of noninferiority to sorafenib and achieved significant and clinically meaningful improvement in objective response rate, progression-free survival, and time to progression .
Genomic analysis of HCC has provided insight into the driver genes or pathways . For instance, a focal amplification on chromosome 11q13 (FGF19 and CCND1) and overexpression of the FGF19 gene has been identified in a subset of HCCs, and multiple studies have demonstrated the roles of the FGF19–FGFR4 axis in HCC . In addition, other studies of HCC preclinical models have shown the importance of FGF signal pathways . Therefore, targeting of FGFR signaling in HCC is attracting a great of attention.
Here, we investigated the antitumor activity of lenvatinib in various preclinical HCC models and explored the mechanisms underlying its antitumor activity.
Materials and Methods
Cell lines and reagents
Hep3B2.1-7, SNU-398, and SNU-449 cells were obtained from the American Type Culture Collection (Manassas, VA); HuH-1, HuH-7, and JHH-7 cells were from Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan); Li-7 and PLC/PRF/5 cells were from the Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University (Miyagi, Japan); and SK-HEP-1 cells were from DS Pharma Biomedical (Tokyo, Japan). Lenvatinib (lenvatinib mesilate) was synthesized at Eisai Co., Ltd. (Ibaraki, Japan). Sorafenib (sorafenib tosylate) was prepared from sorafenib tablets (Bayer Yakuhin, Ltd., Osaka, Japan). Recombinant human FGF19 was obtained from R&D Systems (Minneapolis, MN).
Cell culture
Cell lines were incubated at 37°C in 5% CO2 in the following media containing 10% FBS and 100 units/mL penicillin–100 μg/mL streptomycin: EMEM (Wako, Osaka, Japan) for Hep3B2.1-7; DMEM (Wako) for HuH-7; Williams’ Medium E (Sigma–Aldrich, St. Louis, MO) for JHH-7; high-glucose DMEM (Wako) for SK-HEP-1, HuH-1, and PLC/PRF/5; RPMI-1640 (Wako) for Li-7, SNU-398, and SNU-449; and RPMI-1640 (Invitrogen, Carlsbad, CA) supplemented with 10 μg/mL insulin and 2 μmol/L hydrocortisone, for LIXC-012.
HCC xenograft models
Animal experiments were performed in accordance with the guidelines approved by the Institutional Animal Care and Use Committee of Eisai Co., Ltd., Shanghai ChemPartner Co., Ltd., or Crown Bioscience Inc. Lenvatinib was dissolved in 3 mmol/L HCl. Sorafenib was dissolved in cremophor:ethanol (1:1) and then diluted fourfold with distilled water. HCC cells (2.5 × 106 to 5 × 106) in HBSS (for Hep3B2.1-7 and SNU-398) or culture medium (for PLC/PRF/5) were mixed with Matrigel [1:1] (Corning, Corning, NY) and then inoculated subcutaneously into the right flank of female BALB/c nude mice. When the tumors reached approximately 170–290 mm3, the mice were randomly allocated to treatment groups (Day 1). Lenvatinib, sorafenib, or the corresponding vehicle was given orally to individual mice once daily for the indicated doses and periods in each figure legend.
The LIXC-012 xenograft study was performed at Shanghai ChemPartner Co., Ltd. (Shanghai, China). LIXC-012 cells (2 × 106) in serum-free medium mixed with Matrigel [1:1] (Corning) were inoculated subcutaneously into the right flank of female nu/nu mice. When the tumors reached approximately 80–300 mm3, the mice were randomly allocated to treatment groups (Day 1); mice then orally received lenvatinib, sorafenib, or the corresponding vehicle once daily for 14 days. Multiple mice in each vehicle control group were removed from the study because of excess tumor volume or cachexia-induced body weight loss (BWL) after Day 11.
Patient-derived xenograft (PDX; LI0050 and LI0334) studies were performed at Crown Bioscience Inc. (Taicang, China). Each tumor fragment (diameter, 2–3 mm) was inoculated subcutaneously into the right flank of female BALB/c nude mice. When the tumors reached approximately 130–150 mm3 (Day 1), the mice were randomly allocated to treatment groups; mice then orally received lenvatinib, sorafenib, or vehicle (3 mmol/L HCl only) once daily for 28 days. In the PDX studies, when BWL was ≥20% for any individual mouse, that mouse was given dosing holiday(s) until its body weight recovered to baseline (BWL, ≤10%).
Tumor dimensions were measured two or three times weekly with a caliper, and tumor volume was calculated as ½ × length × width2. Relative body weight (RBW) was calculated by dividing the daily body weight by that of the same mouse on Day 1.
Western blot analysis
Cells were washed and lysed with RIPA buffer containing protease inhibitor cocktail (Roche Diagnostics, Mannheim, Germany) and Halt phosphatase inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA). To examine the effect on FGF signaling pathways in vitro, HCC cells were treated with lenvatinib, sorafenib, or vehicle (DMSO) for 1 h before cell lysis. In the case of Hep3B2.1-7, the cells were starved overnight in culture medium containing 0.5% BSA; incubated with each drug for 1 h; treated with 100 ng/mL FGF19 for 5 min; and then washed and lysed. To examine the inhibitory effect on FGF signaling pathways in vivo, mice bearing xenograft tumors underwent single treatment with lenvatinib, sorafenib, or the corresponding vehicle; the tumors were collected 2 h later; and the samples were homogenized and lysed.
Cell and tumor lysates were cleared by centrifugation, denatured in 1× Laemmli sample buffer, separated by SDS-PAGE, and transferred to PVDF membranes. The membranes were incubated with antibodies to FGFR1, FGFR3, FGFR4, p-FRS2 (Tyr436), Erk1/2, and p-Erk1/2 (Cell Signaling Technology, Beverly, MA), FGFR2 [Bek] (Santa Cruz Biotechnology, Dallas, TX), KLB (β-Klotho) and FRS2 (R&D Systems), β-actin and FGF19 (Sigma-Aldrich) at the recommended concentrations. Bands were visualized as described previously .
In vitro proliferation assay
Human HCC cells were plated on 96-well plates at 1 × 103 cells/well and cultured at 37°C in 5% CO2. Next day, the cells were treated with 1:2 serial dilutions of lenvatinib, sorafenib, or vehicle (DMSO), and cultured for 6 days. Cell viability was measured by using a Cell Counting Kit-8 (Dojindo Molecular Technologies, Kumamoto, Japan), and IC50 values were determined using GraphPad Prism (GraphPad Software, La Jolla, CA). Each experiment contained triplicate samples, and three independent experiments were performed.
Immunohistochemistry
Xenograft tumors were collected on the day after the last dosing; tumor fragments were fixed with 10% formalin and embedded in paraffin, except for LI0334 tumor fragments, which were frozen in OCT compound and the tissue sections were fixed with cold acetone. Staining for endothelial cells with anti-CD31 antibody and microvessel density (MVD) measurement were performed as described previously . Briefly, tissue sections were stained with anti-CD31 antibody (Dianova, Hamburg, Germany) and the slides were scanned using the Aprio ScanScope XT system. Five regions of interest (ROIs, each 500 × 500 μm) with the highest densities of CD31-stained microvessels were selected manually, and the number of microvessels in each ROI was measured.
Ki-67-positive cells were stained with anti-Ki-67 antibody (Cell Signaling Technology) and detected using Envision+ Single Reagents (Dako, Agilent Technologies, Santa Clara, CA). The percentage of Ki-67 positive nuclei was quantified using HALO software (Indica Labs, Corrales, NM).
Statistical analysis
Unless otherwise indicated, tumor volume, MVD, and Ki-67-positive cells (%) were compared between treatment groups using the Dunnett's multiple comparison test. P values <0.05 were considered significant.
Additional Materials and Methods are provided in Appendix S1 and Tables S1–S3.
Results
Antiproliferative activity of lenvatinib against HCC cell lines
Activation of the FGF signaling pathways is associated with HCC progression, and proliferation of subsets of HCC is dependent on FGF signaling pathways . Because lenvatinib inhibits FGFRs, we first examined the antiproliferative activity of lenvatinib against nine HCC cell lines in vitro. Lenvatinib showed selective and potent antiproliferative activity against the HCC cell lines Hep3B2.1-7, HuH-7, and JHH-7, with IC50 values of 0.23, 0.42, and 0.64 μmol/L, respectively (Fig. A and B). Immunoblotting analysis showed that FGF19, FGFR4, and β-Klotho were highly expressed in these three cell lines, indicating that the FGF19–FGFR4 axis was activated (Fig. C), consistent with previous reports . Lenvatinib also showed moderate inhibitory effects on the proliferation of SNU-398, Li-7, and HuH-1 cells, with IC50 values of 1.56, 1.65, and 2.59 μmol/L, respectively (Fig. A and B). Although FGF19 protein was not expressed, some members of the FGFR family were detected in these cells (Fig. C). Because these cell lines are modestly sensitive to pan-FGFR inhibitors such as BGJ398 , lenvatinib might inhibit proliferation of the cells by targeting an activated FGF signaling pathway. Lenvatinib did not show clear antiproliferative activity against SK-HEP-1, SNU-449, or PLC/PRF/5 cells (IC50 >5 μmol/L; Fig. A and B), which were also insensitive to pan-FGFR inhibitors .
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As a reference, we also examined the antiproliferative activity of sorafenib, an approved systemic treatment drug for uHCC , in the nine HCC cell lines. Sorafenib did not show selective antiproliferative activity against HCC cell lines with high FGF19 expression (Fig. ). These results indicate that lenvatinib selectively suppressed the proliferation of HCC cells with activated FGF signaling pathways.
Lenvatinib binds to the receptor tyrosine kinase VEGFR2 with Type V binding mode ; this may contribute to its high affinity and its selectivity for a small number of receptor tyrosine kinases. Co-crystal analysis with lenvatinib–FGFR1 complex revealed that lenvatinib also bound to FGFR1 with Type V binding mode (Fig. S1). Homology modeling and docking simulation based on the lenvatinib–FGFR1 complex suggest that the mode of binding to FGFR2, 3, and 4 is identical to that to FGFR1 (Figs S2 and S3).
Inhibitory effect of lenvatinib on FGF signaling pathways in HCC cell lines
We next examined the effects of lenvatinib on FGF signaling pathways in the HCC cell lines by Western blot analysis. In Hep3B2.1-7 and HuH-7 cells, which are highly expressing FGF19, constitutive phosphorylation of FRS2, an FGFR downstream signaling molecule, was observed; in Hep3B2.1-7 cells, this phosphorylation was further enhanced by the addition of recombinant human FGF19. Lenvatinib suppressed the phosphorylation of FRS2 at 0.03–3 μmol/L in both cell types (Fig. A and B). The IC50 values of lenvatinib in the proliferation assay against these cells were parallel to the concentrations of lenvatinib for pFRS2 inhibition. Furthermore, lenvatinib dose dependently inhibited FRS2 phosphorylation in SNU-398 cells (Fig. C). Lenvatinib inhibited the basic FGF (bFGF)-stimulated phosphorylation of FRS2 in SNU-449 cells (Fig. S4), which express high levels of FGFR1, although lenvatinib did not show potent antiproliferative activity against the cells (Fig. ). In contrast, sorafenib did not show clear inhibition of FRS2 phosphorylation among the HCC cell lines tested. These results suggest that lenvatinib-mediated blockade of FGFRs in HCC cells with activated FGF signaling pathways is the likely basis of the antiproliferative activity of this compound in vitro.
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Antitumor activity of lenvatinib against xenograft models with activated FGF signaling pathways
We next evaluated the in vivo antitumor activity of lenvatinib against HCC xenografts with activated FGF signaling pathways in the Hep3B2.1-7 and SNU-398 models. In vivo growth of xenograft tumors was significantly inhibited by lenvatinib at doses of 3–30 mg/kg in the Hep3B2.1-7 model and 10, 30 mg/kg in the SNU-398 model, and by sorafenib at 10 and 30 mg/kg in the Hep3B2.1-7 model and 30 mg/kg in the SNU-398 model (Fig. A and B). BWL of the treatment groups was similar to that of the corresponding vehicle control group, although cachexia-induced BWL was observed in each control group (Fig. S5).
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To examine whether lenvatinib inhibited FGF signaling pathways within the Hep3B2.1-7 and SNU-398 xenograft tumors, tumor samples were collected 2 h after a single lenvatinib treatment, and phosphorylation levels of FRS2 and another downstream molecule of FGFR, Erk1/2, were evaluated. Lenvatinib at 10 and 30 mg/kg inhibited phosphorylation of FRS2 and Erk1/2 in the Hep3B2.1-7 model (Fig. C); lenvatinib at 3–30 mg/kg inhibited FRS2 phosphorylation in the SNU-398 model (Fig. E); and lenvatinib at 30 mg/kg inhibited Erk1/2 phosphorylation in the SNU-398 model (Fig. E). In contrast, no clear suppression was observed in sorafenib-treated xenograft tumors in either model (Fig. D and F). Inhibition of the FGF signaling pathway in vivo was also observed in human HCC HuH-7 xenografts, which overexpressed FGF19 (Fig. S6). These results suggest that targeting FGFR of HCC cells underlies the antitumor activity of lenvatinib in preclinical HCC models with activated FGF signaling pathways.
Antitumor activity of lenvatinib against PLC/PRF/5 xenograft model
In contrast to Hep3B2.1-7, HuH-7, and SNU-398 cells, PLC/PRF/5 cells were not sensitive to lenvatinib and other FGFR inhibitors in vitro and do not overexpress FGF19, even though FGFR4 is expressed (Fig. ), suggesting that proliferation of PLC/PRF/5 cells was not dependent on an activated FGF signaling pathway. Lenvatinib inhibited in vivo tumor growth of PLC/PRF/5 xenografts at all doses tested (Fig. A), and sorafenib also inhibited in vivo tumor growth at 30 mg/kg (Fig. A). No obvious BWL was noted in either treatment group compared with its corresponding control group (Fig. S7). In an additional experiment, the maximum antitumor activity of lenvatinib was greater than that of sorafenib in the PLC/PRF/5 model (Fig. S8). To examine whether the antitumor activity of lenvatinib and sorafenib against PLC/PRF/5 xenografts was based on angiogenesis inhibition, we measured MVD within PLC/PRF/5 xenograft tumors. In accordance with the antitumor activity, tumor MVD was significantly decreased by Day 8 following lenvatinib treatment at 3–30 mg/kg and sorafenib treatment at 30 mg/kg (Fig. B and C). These results suggest that lenvatinib can exert antitumor activity against HCC tumors whose proliferation is not dependent on an FGF signaling pathway, via its anti-angiogenic effect.
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We also measured MVD in the Hep3B2.1-7 and SNU-398 xenograft models after treatment with lenvatinib and sorafenib. Lenvatinib (3–30 mg/kg for Hep3B2.1-7 model; 10 and 30 mg/kg for SNU-398 model) and sorafenib (30 mg/kg for both models) significantly reduced MVD in xenograft tumors (Fig. S9), suggesting that lenvatinib could target the FGFR of HCC cells and tumor angiogenesis in the preclinical HCC models with activated FGF signaling pathways, whereas sorafenib targeted mainly tumor angiogenesis or an undefined signaling pathway.
Antitumor activity of lenvatinib in a xenograft model of patient-derived xenograft (PDX)-derived cell line and two HCC PDX models
We examined the antitumor activity of lenvatinib using a xenograft model of PDX-derived cell line and two HCC PDX models. PDX LIX-012 robustly expresses FGF19, although it does not possess FGF19 gene amplification . The LIXC-012 cell line, which was established from PDX LIX-012 , overexpresses FGF19, FGFR4, and KLB and is sensitive to BLU9931, a selective FGFR4 inhibitor, suggesting that FGF19-activated FGF signaling plays a role in LIXC-012 models . Here, lenvatinib at 3–30 mg/kg and sorafenib at 30 mg/kg inhibited the in vivo growth of LIXC-012 xenograft tumors (Fig. A). Cachexia-induced BWL was observed in this model, but mice treated with lenvatinib at 10 and 30 mg/kg showed recovery of BWL (Fig. S10). To examine whether lenvatinib inhibits LIXC-012 cell proliferation in vivo, we stained for the proliferation marker Ki-67 within xenograft tumors collected 1 day after the last lenvatinib dose. Lenvatinib treatment at 3–30 mg/kg significantly reduced the number of Ki-67-positive cells (Fig. B). A significant but modest inhibition of Ki-67 staining was observed with sorafenib treatment at 30 mg/kg only (Fig. B).
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We also examined the inhibitory effect of lenvatinib on tumor growth in two HCC PDX models, LI0050 and LI0334. Lenvatinib at 10 and 30 mg/kg inhibited the tumor growth of both PDXs, and this was accompanied by a dramatic and significant decrease in MVD (Fig. ). Sorafenib at 30 mg/kg was not tolerated in the LI0050 model and failed to show antitumor and anti-angiogenic activities in the LI0334 model (Fig. ). In both models, BWL in the lenvatinib treatment groups was comparable to that in the vehicle control group (Fig. S11). These results indicate that lenvatinib had antitumor activity against HCC PDX models, likely through its potent anti-angiogenic activity. Collectively, the antitumor activity of lenvatinib was more robust than that of sorafenib in a wide variety of preclinical HCC models, including cell line xenograft and PDX models (Fig. S12).
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Discussion
Lenvatinib is an inhibitor of multiple tyrosine kinases including VEGFRs and FGFRs . In particular, the potent activity against FGFR1–4 is a distinctive feature of lenvatinib, compared with sorafenib. In addition to kinase profile, the binding mode with target kinases differs between the two drugs: lenvatinib binds to VEGFR2 in a Type V binding mode, whereas sorafenib has a Type II binding mode . Here, we discovered that lenvatinib also binds to FGFR1 in a Type V binding mode, and homology modeling and docking simulations suggest that the mode of binding to FGFR2, FGFR3, and FGFR4 is identical to that to FGFR1 (Figs S1–S3). Thus, these differences in molecular characteristics of lenvatinib and sorafenib may underlie the differences in the pharmacological activities and mechanisms of action of these drugs.
Increasing evidence suggests that activation of FGF signaling pathways in HCC contributes to its malignancy . Therefore, we examined the antiproliferative activity of lenvatinib against nine HCC cell lines, and we showed that lenvatinib has inhibitory activity against HCC cell lines with activated FGF signaling pathways ; this proliferation inhibition was accompanied by suppressed FRS2 phosphorylation (Figs and ). These results indicate that the selective antiproliferative activity of lenvatinib was based on inhibition of activated FGF signaling pathways. Inhibition of FGF signaling by lenvatinib was also observed in xenograft tumors (Fig. ). Further study is warranted to investigate how an inhibitory effect of lenvatinib on tumor FGF signaling pathways contributes to its antitumor activity in the HCC models. Sorafenib has inhibitory activity against RAF kinase acting downstream of receptor tyrosine kinases and upstream of ErK1/2. However, in the current study, sorafenib showed neither selectivity against the proliferation of HCC cell lines with the activated FGF pathway nor downregulation of p-Erk1/2 in xenograft tumors (Figs and ), suggesting that sorafenib may not affect FGF signaling pathways in these preclinical HCC models.
Autocrine activation of FGF signaling involving FGFR4 and its cognate ligand FGF19 is reported in about one-third to one-half of HCC patients . Therefore, targeting FGFR4 is expected to be an effective approach for treatment of uHCC. Indeed, a selective FGFR4 inhibitor has recently been shown in a phase 1 clinical trial of patients with advanced HCC to provide objective clinical activity in FGF19-positive disease . Here, we showed that lenvatinib inhibited tumor growth and suppressed the FGF signaling pathway in FGF19-overexpressing HCC xenograft models. In contrast, sorafenib did not affect the FGF signaling pathway in vitro and in vivo, suggesting that the mode of actions of the two drugs differs in HCC models with FGF19 overexpression.
In addition to the FGF19–FGFR4 axis, another FGF–FGFR axis might affect cell proliferation and in vivo tumor growth of HCC xenograft tumors . In preclinical experiments using HCC PDX models, lenvatinib had a greater response than sorafenib in those models highly expressing FGFR1 mRNA . In our study, lenvatinib inhibited the proliferation of SNU-398, Li-7, and HuH-1 cells in vitro (Fig. A and B), all of which did not produce FGF19 (Fig. C) but are known to be sensitive to FGFR inhibitors . Given that these HCC cell lines expressed multiple FGFRs (Fig. C), further experiments are required to delineate the FGFR(s) responsible for tumor growth. Most importantly, our results suggest that lenvatinib can have a direct antiproliferative effect on HCC cells with an activated FGF signaling pathway that does not involve the FGF19–FGFR4 axis. Long-term exposure to sorafenib confers sorafenib resistance on HCC cells with upregulation of FGFR1 . Considering this result, lenvatinib has the potential to overcome such resistance by blocking FGFRs.
Because HCCs are highly angiogenic, anti-angiogenic therapy is a promising approach to the treatment of uHCC . Here, lenvatinib inhibited tumor angiogenesis in all HCC xenograft models tested, regardless of the activation status of FGF signaling pathways, suggesting that inhibition of angiogenesis underlies the antitumor activity of lenvatinib in HCC models. The anti-angiogenic activity of lenvatinib was more potent than that of sorafenib, especially in PDX models (Fig. ). Accumulating evidence indicates that FGF acts as a pro-angiogenic factor and induces escape from anti-VEGF therapy . Consistently, anti-bFGF monoclonal antibody inhibits angiogenesis and has additive effects in combination with sorafenib in HCC xenograft models . We previously revealed that lenvatinib targets VEGF- and FGF-induced angiogenesis , and therefore the blocking of not only VEGFR but also FGFR by lenvatinib might be behind its potent anti-angiogenic and antitumor activities in the HCC models.
Recently, an anti-programmed death-1 (PD-1) antibody, nivolumab, provided clinically meaningful responses in patients with uHCC in a phase 1/2 study (NCT01658878), suggesting that tumor immune regulation also plays an important role in the antitumor effect in uHCC . Interestingly, VEGF modulates immune responses and targeting VEGF improved the activity of anti-PD-1 treatment in preclinical models . In addition, FGFR3 activation is associated with low T-cell infiltration in bladder cancer, and the FGF signaling pathway participates in tumor immunity . Considering these findings, further studies are crucial to elucidate the effect of lenvatinib-mediated dual blockade of VEGFR and FGFR on tumor immunity in HCC models. These studies may provide insights into the antitumor activity and molecular mechanisms of lenvatinib in the clinical setting.
In conclusion, we demonstrated that lenvatinib exerts robust antitumor activity against various HCC models, including cell line xenograft and PDX models, and that blockade of activated FGF signaling pathways in HCC tumor cells and potent anti-angiogenic activity underlie these antitumor activities.
Acknowledgments
The authors thank Sunplanet Co., Ltd. for performing in vitro and in vivo studies; Shanghai ChemPartner Co., Ltd. and Crown Bioscience Inc. for performing in vivo studies; Proteros biostructures GmbH for performing crystallographic studies and helpful support; and Masayuki Okada, Kotaro Kodama for reviewing the manuscript.
Conflict of Interest
All authors are employees of Eisai Co., Ltd.
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Copyright John Wiley & Sons, Inc. 2018
Abstract
Unresectable hepatocellular carcinoma (
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; Hoshi, Taisuke 1 ; Yamamoto, Yuji 1 ; Ikemori‐Kawada, Megumi 1 ; Minoshima, Yukinori 1 ; Funahashi, Yasuhiro 1 ; Matsui, Junji 1 1 Tsukuba Research Laboratories, Eisai Co., Ltd., Ibaraki, Japan