Effects of Puerarin Derivative P on Learning, Memory and MPO Activity in Vascular Dementia Model Mice

Abstract

Pharmacological experiments confirmed that Puerarin could not only expand the arterial vessels, increase the local blood flow, but also could protect the myocardium, improve the blood supply of ischemic tissue, which was used to treat the cerebrovascular dementia disease in clinical practice. Its own structure particularity caused the lower bioavailability and poorer solubility. In order to improve the deficiency, the related pharmacological experiments of the newly synthesized puerarin derivative (P), so as to expect the better curative effect than puerarin in the process of treating vascular dementia. The vascular dementia model was established by permanently ligating the common carotid artery in mice. The effects of puerarin derivative (P) on learning and memory in mice by water maze, Y maze and new object discrimination methods; The myeloperoxidase activity in the ischemic cerebral cortex was evaluated in mice by biochemical method. The experimental results showed that the mice spontaneous alternation response accuracy in Y maze could be obviously improved in 100mg/kg puerarin derivative group; In water maze, the swimming time to the safe platform would be significantly decreased in puerarin derivative group. Meanwhile, the MPO activity in the cerebral cortex of dementia mice was significantly decreased in 100mg/kg puerarin derivative group by permanently ligating the unilateral common carotid artery.