Abstract

This work, as a proof of principle, presents a sensitive and selective electrochemical immunosensor for Zika-virus (ZIKV)-protein detection using a functionalized interdigitated micro-electrode of gold (IDE-Au) array. A miniaturized IDE-Au immunosensing chip was prepared via immobilization of ZIKV specific envelop protein antibody (Zev-Abs) onto dithiobis(succinimidyl propionate) i.e., (DTSP) functionalized IDE-Au (electrode gap/width of 10 µm). Electrochemical impedance spectroscopy (EIS) was performed to measure the electrical response of developed sensing chip as a function of ZIKV-protein concentrations. The results of EIS studies confirmed that sensing chip detected ZIKV-protein selectively and exhibited a detection range from 10 pM to 1 nM and a detection limit of 10 pM along with a high sensitivity of 12 kΩM−1. Such developed ZIKV immune-sensing chip can be integrated with a miniaturized potentiostat (MP)-interfaced with a smartphone for rapid ZIKV-infection detection required for early stage diagnostics at point-of-care application.

Details

Title
A sensitive electrochemical immunosensor for label-free detection of Zika-virus protein
Author
Kaushik, Ajeet 1 ; Yndart, Adriana 1 ; Kumar, Sanjeev 2 ; Jayant, Rahul Dev 1   VIAFID ORCID Logo  ; Vashist, Arti 1 ; Brown, Ashley N 3 ; Chen-Zhong, Li 4 ; Nair, Madhavan 1 

1  Center of Personalized Nanomedicine, Institute of NeuroImmune Pharmacology, Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, Miami, Florida, USA 
2  Biomedical Instrumentation, CSIR-Central Scientific Instruments Organization, Sector 30-C, Chandigarh, India 
3  Institute for Therapeutic Innovation, Department of Medicine, College of Medicine, University of Florida, Orlando, Florida, USA 
4  Nanobioengineering/Bioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, Miami, Florida, USA 
Pages
1-5
Publication year
2018
Publication date
Jun 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-018-28035-3
ProQuest document ID
2059536775
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.