Lapine periodontal ligament stem cells for musculoskeletal research in preclinical animal trials

Abstract

STRO-1+CD146+ hPDLSCs were isolated from hPDL cells but not from the rPDL cells. [...]heterogeneous population of rabbit and human PDL cells were subsequently used for latter comparative studies. Periodontal regeneration is always challenging due to the complexity of the structure of periodontium and heterogeneity of cells. [...]there is a paradigm shift to explore using stem cell-based regenerative therapies as an alternate treatment modality for periodontal regeneration. On the contrary, we were able to isolate STRO-1+/CD146+ hPDLSCs (data not shown) and the proportion of which is similar to the previous studies [10, 11]. [...]to make our study comparable with the human cells, we used the heterogeneous population of both PDL cells in all sets of downstream experiments. [...]despite of being heterogenous in nature, our results showed that the PDL cells demonstrated certain properties of MSC cells but to a lesser extent. [...]studies should be done to validate the potential of using rPDL cells instead of rPDLSCs in stem cell research.