Prevalence of human pathogenic Yersinia enterocolitica in Swedish pig farms

Abstract

Methods Assessment of culture method for detection of Yersinia enterocolitica in pig faeces For enumeration, two broths prepared in-house were used: phosphate-buffered saline containing 2% sorbitol and 0.15% bile salts (PSB) and phosphate-buffered saline with 0.5% peptone, 1% mannitol and 0.15% bile salts (PMB).Suspected Y. enterocolitica colonies were streaked onto a blood agar plate, incubated at 25 °C for 24 h and confirmed as Y. enterocolitica using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry, MALDI-TOF (Bruker Daltonics, Bremen, Germany) according to the manufacturer’s instructions.Risk factor analysis The correlation between production practices recorded on the survey at the pen and herd levels and the odds of detection of Y. enterocolitica in the collected faecal samples was tested by mixed variable logistic regression in the lme4 package (version 1.1–12) for R (version 3.2.2).Infect Ecol Epidemiol. 2012;2:19027.View ArticleGoogle Scholar Rizzardi K, Wahab T, Jernberg C. Rapid subtyping of Yersinia enterocolitica by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for diagnostics and surveillance.