Abstract

Red-shanked doucs (Pygathrix nemaeus) are endangered, foregut-fermenting colobine primates which are difficult to maintain in captivity. There are critical gaps in our understanding of their natural lifestyle, including dietary habits such as consumption of leaves, unripe fruit, flowers, seeds, and other plant parts. There is also a lack of understanding of enteric adaptations, including their unique microflora. To address these knowledge gaps, we used the douc as a model to study relationships between gastrointestinal microbial community structure and lifestyle. We analyzed published fecal samples as well as detailed dietary history from doucs with four distinct lifestyles (wild, semi-wild, semi-captive, and captive) and determined gastrointestinal bacterial microbiome composition using 16S rRNA sequencing. A clear gradient of microbiome composition was revealed along an axis of natural lifestyle disruption, including significant associations with diet, biodiversity, and microbial function. We also identified potential microbial biomarkers of douc dysbiosis, including Bacteroides and Prevotella, which may be related to health. Our results suggest a gradient-like shift in captivity causes an attendant shift to severe gut dysbiosis, thereby resulting in gastrointestinal issues.

Details

Title
Associations Between Nutrition, Gut Microbiome, and Health in A Novel Nonhuman Primate Model
Author
Clayton, Jonathan B 1   VIAFID ORCID Logo  ; Al-Ghalith, Gabriel A 2 ; Long, Ha Thang 3 ; Bui Van Tuan 4 ; Cabana, Francis 5   VIAFID ORCID Logo  ; Huang, Hu 2 ; Vangay, Pajau 6 ; Ward, Tonya 7 ; Vo Van Minh 8 ; Nguyen Ai Tam 4 ; Nguyen, Tat Dat 4 ; Travis, Dominic A 9 ; Murtaugh, Michael P 10 ; Covert, Herbert 11 ; Glander, Kenneth E 12   VIAFID ORCID Logo  ; Nadler, Tilo 13 ; Toddes, Barbara 14 ; Sha, John C M 15 ; Singer, Randy 10 ; Knights, Dan 16 ; Johnson, Timothy J 17 

 Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, MN, USA; GreenViet Biodiversity Conservation Center, Son Tra District, Da Nang, Vietnam; Primate Microbiome Project, Minneapolis, MN, USA 
 Biomedical Informatics and Computational Biology, 200 Union St SE, University of Minnesota, Minneapolis, MN, USA 
 GreenViet Biodiversity Conservation Center, Son Tra District, Da Nang, Vietnam; Primate Microbiome Project, Minneapolis, MN, USA; Frankfurt Zoological Society, Frankfurt, Germany 
 GreenViet Biodiversity Conservation Center, Son Tra District, Da Nang, Vietnam 
 Primate Microbiome Project, Minneapolis, MN, USA; Wildlife Nutrition Centre, Wildlife Reserves Singapore, Singapore, Singapore 
 Biomedical Informatics and Computational Biology, 200 Union St SE, University of Minnesota, Minneapolis, MN, USA; Biotechnology Institute, University of Minnesota, Saint Paul, MN, USA 
 Biotechnology Institute, University of Minnesota, Saint Paul, MN, USA 
 Faculty of Biology and Environmental Science, The University of Da Nang - University of Science and Education, Lien Chieu District, Da Nang, Vietnam 
 Primate Microbiome Project, Minneapolis, MN, USA; Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, MN, USA 
10  Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, MN, USA 
11  Department of Anthropology, University of Colorado Boulder, Boulder, CO, USA 
12  Primate Microbiome Project, Minneapolis, MN, USA; Department of Evolutionary Anthropology, Duke University, Durham, NC, USA 
13  Endangered Primate Rescue Center, Cuc Phuong National Park, Nho Quan District, Ninh Binh Province, Vietnam 
14  Philadelphia Zoological Garden, Philadelphia, PA, USA 
15  School of Sociology and Anthropology, Sun Yat-Sen University, Guangzhou, China 
16  Primate Microbiome Project, Minneapolis, MN, USA; Biomedical Informatics and Computational Biology, 200 Union St SE, University of Minnesota, Minneapolis, MN, USA; Biotechnology Institute, University of Minnesota, Saint Paul, MN, USA; Department of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, USA 
17  Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, MN, USA; Primate Microbiome Project, Minneapolis, MN, USA; University of Minnesota, Mid-Central Research and Outreach Center, Willmar, Minnesota, USA 
Pages
1-16
Publication year
2018
Publication date
Jul 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2075523500
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.