Validation of a simplex PCR assay enabling reliable identification of clinically relevant Candida species

Abstract

The rapid and correct identification of Candida species can narrow therapy options by preventing treatment with potentially toxic antifungal agents, thus reducing costs of hospitalization and improving negative patient outcomes [24–26]. Because of the phenotypic similarities of Candida species, the turn-around time of traditional, culture based identification methods may take 2 to 8 days, delay adequate diagnosis and appropriate antifungal treatment. [...]the grand mean of the sample coefficient of variations (average % C.V.-s) of the three plates was taken defining the plate % C.V. values. [...]grand mean of the sample coefficient of variations (average % C.V.-s) was taken. [...]intra- and inter-assay consistencies were calculated.