Sotetsuflavone inhibits proliferation and induces apoptosis of A549 cells through ROS-mediated mitochondrial-dependent pathway

Abstract

Sotetsuflavone had the strongest inhibitory effect on A549 cells. [...]in order to clarify the effect of Sotetsuflavone on A549 cells, we studied its potential molecular mechanism, and evaluated whether Sotetsuflavone can be safely utilized by humans as therapeutic agent. The intracellular reactive oxygen species can convert non-fluorescent DCFH into fluorescent DCF [22]. [...]the fluorescence intensity of DCF can reflect the level of ROS [23]. A flow cytometry assay showed that treatment with sotetsuflavone for 24 h resulted apoptosis in A549 cells. [...]the proportion of apoptotic cells was significantly increased with elevating levels of drug concentrations (Figs. 2 and 3b). [...]sotetsuflavone may be used as a novel candidate for anti-tumor therapy in patients with lung cancer.