Abstract

Dielectrophoresis using multi-electrode arrays allows a non-invasive interface with biological cells for long-term monitoring of electrophysiological parameters as well as a label-free and non-destructive technique for neuronal cell manipulation. However, experiments for neuronal cell manipulation utilizing dielectrophoresis have been constrained because dielectrophoresis devices generally function outside of the controlled environment (i.e. incubator) during the cell manipulation process, which is problematic because neurons are highly susceptible to the properties of the physiochemical environment. Furthermore, the conventional multi-electrode arrays designed to generate dielectrophoretic force are often fabricated with non-transparent materials that confound live-cell imaging. Here we present an advanced single-neuronal cell culture and monitoring platform using a fully transparent microfluidic dielectrophoresis device for the unabated monitoring of neuronal cell development and function. The device is mounted inside a sealed incubation chamber to ensure improved homeostatic conditions and reduced contamination risk. Consequently, we successfully trap and culture single neurons on a desired location and monitor their growth process over a week. The proposed single-neuronal cell culture and monitoring platform not only has significant potential to realize an in vitro ordered neuronal network, but also offers a useful tool for a wide range of neurological research and electrophysiological studies of neuronal networks.

Details

Title
Single-neuronal cell culture and monitoring platform using a fully transparent microfluidic DEP device
Author
Kim, Hyungsoo 1 ; Lee, In-Kyu 1 ; Taylor, Kendra 2 ; Richters, Karl 3 ; Dong-Hyun Baek 4 ; Jae Ha Ryu 1 ; Cho, Sang June 1   VIAFID ORCID Logo  ; Jung, Yei Hwan 1 ; Park, Dong-Wook 5 ; Novello, Joseph 4 ; Bong, Jihye 1 ; Suminski, Aaron J 4 ; Dingle, Aaron M 6 ; Blick, Robert H 1 ; Williams, Justin C 4   VIAFID ORCID Logo  ; Dent, Erik W 3 ; Ma, Zhenqiang 1 

 Department of Electrical and Computer Engineering, University of Wisconsin–Madison, Madison, WI, USA 
 Neuroscience Training Program, University of Wisconsin-Madison, Madison, WI, USA 
 Department of Neuroscience, University of Wisconsin-Madison, Madison, WI, USA 
 Department of Biomedical Engineering, University of Wisconsin–Madison, Madison, WI, USA 
 Department of Electrical and Computer Engineering, University of Wisconsin–Madison, Madison, WI, USA; School of Electrical and Computer Engineering, University of Seoul, Seoul, South Korea 
 Department of Surgery, University of Wisconsin-Madison, Madison, WI, USA 
Pages
1-9
Publication year
2018
Publication date
Sep 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2099430670
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.