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Background: Extracellular vesicles (EVs) represent versatile entities with body-wide signalling functions as they pass barriers and deliver complex biomolecules between cells and tissues. We recently demonstrated that exosome-like EVs are secreted into the circulation during an early phase of exercise. Physical activity is known to exhibit a wide range of beneficial properties regarding cardiovascular and immune functions as well as the ageing process. Determination of the source and target cell populations is essential to examine the potential systemic effects...