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* Background.-Uterine endometrial stromal sarcoma is a rare neoplasm with a morphology that closely resembles that of the proliferative endometrial stroma. To understand its pathologic characteristics, we established a novel cell line, MaMi, from a primary culture of an endometrial stromal sarcoma obtained from a 65-year-old Japanese woman.
Methods.-We observed the morphology of MaMi cells and performed immunohistochemical analysis on the primary tumor and transplants in nude mice. Prolactin, insulin-like growth factor-binding protein-1, interleukin (IL)6, IL-8, monocyte chemoattractant protein-1, intercellular adhesion molecule-1, E-selectin, vascular cell adhesion molecule-1, and fibronectin production in the culture medium of MaMi cells were also examined.
Results.-MaMi cells were shown to exhibit a fibroblastlike morphology in vitro, and they adopted a more elongated appearance in response to 12-O-tetradecanoyl phorbol-13-acetate (TPA). On injection into nude mice, the cells gave rise to subcutaneous tumors. Immunohistologi
cally, both the primary tumor and MaMi cell-induced tumors stained positively with antibodies to neuron-specific enolase or vimentin. MaMi cells constitutively produced IL-6, IL-8, and monocyte chemoattractant protein-1 in vitro. Interleukin-1 1 (100 pmol/L), tumor necrosis factor-a (1 nmol/L), and lipopolysaccharide (1 (mu)g/mL) each increased the release of IL-6, IL-8, and monocyte chemoattractant protein-1 by MaMi cells. TPA (10 nmol/L) also stimulated the production of IL-6 and IL-8 by these cells, but inhibited that of monocyte chemoattractant protein-1.
Conclusions.-We demonstrated that MaMi cells closely resemble proliferative endometrial stromal cells not only morphologically, but also functionally. This cell line may prove valuable in understanding the role of cytokines produced by tumor cells in the pathogenesis of endometrial stromal sarcoma and may also be useful as an in vitro model of functioning endometrial stromal cells. (Arch Pathol Lab Med. 1998;122:836-841)
Uterine endometrial stromal sarcoma is a rare neoplasm with a morphology that closely resembles that of the proliferative endometrial stroma. It constitutes approximately 0.2% of all uterine malignancies and 10% of all uterine sarcomas.1
Normal endometrial stromal cells produce several bioactive molecules, including interleukin (IL)-6,2,3 prolactin,4 insulin-like growth factor-binding protein-1 (IGFBP1),5,6 as well as fibronectin and laminin,7 especially after decidualization. Intercellular adhesion molecule-1 (ICAM1),8,9 vascular cell adhesion molecule-1 (VCAM-1),8 and IL810 are also expressed by stromal cells.
We now describe the establishment of a cell line, designated MaMi according to the patient's name, from a primary culture of...