Abstract

Over the last decades medicine has developed tremendously, but still many diseases are incurable. The last years, cellular (gene) therapy has become a hot topic in biomedical research for the potential treatment of cancer, AIDS and diseases involving cell loss or degeneration. Here, we will focus on two major areas within cellular therapy, cellular immunotherapy and stem cell therapy, that could benefit from the introduction of neo-expressed genes through mRNA electroporation for basic research as well as for clinical applications. For cellular immunotherapy, we will provide a state-of-the-art on loading antigen-presenting cells with antigens in the mRNA format for manipulation of T cell immunity. In the area of stem cell research, we will highlight current gene transfer methods into adult and embryonic stem cells and discuss the use of mRNA electroporation for controlling guided differentiation of stem cells into specialized cell lineages.

Details

Title
Messenger RNA electroporation: an efficient tool in immunotherapy and stem cell research
Author
A Van Driessche; Ponsaerts, P; Van Bockstaele, D R; V F I Van Tendeloo; Berneman, Z N
Pages
213-6
Section
Review article
Publication year
2005
Publication date
2005
Publisher
Wydawnictwo Via Medica
ISSN
02398508
e-ISSN
18975631
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
213078490
Copyright
Copyright Folia Histochemica et Cytobiologica 2005