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Bulletin of Experimental Biology and Medicine, Vol. 139, No. 5, 2005 GENERAL PATHOLOGY AND PATHOPHYSIOLOGY543Mechanisms of Regulation of Erythropoiesisduring Experimental NeurosesE. G. Skurikhin, A. M. Dygai, N. V. Provalova,M. Yu. Minakova, and N. I. SuslovTranslated from Byulleten Eksperimentalnoi Biologii i Meditsiny, Vol. 139, No. 5, pp. 495-501, May, 2005
Original article submitted June 2, 2004We studied the mechanisms of erythropoiesis regulation in a conflict situation and during
paradoxical sleep deprivation. Plastic reconstruction of the erythroid hemopoietic stem during
experimental neuroses is regulated by a complex multicomponent and multilevel system. This
system consists of central adrenergic structures, sympathetic part of the autonomic nervous
system, and - and -adrenoceptors on erythroid precursors and cells of the hemopoietic
microenvironment. Erythroid cells receive instructive information from the central nervous
system via adrenoceptors on cells of the hemopoiesis-inducing microenvironment and erythroid precursors. Hyperplasia (conflict situation) and suppression of erythropoiesis (paradoxical sleep deprivation) are associated with specificity of central adrenergic regulatory
mechanisms.Key Words: adrenergic system; hemopoiesis-inducing microenvironment; erythropoiesis;
regulationNeuroses and neurotic states contribute to the development of somatic disturbances in various functional
systems of the organism. Experimental neuroses are
always accompanied by changes in the erythron system [4]. Individual components of the system responsible for local regulation of the erythron underwent
changes in a conflict situation (CS) and during paradoxical sleep deprivation (PSD) [3]. Published data
show that the central nervous system (CNS) plays a
role in the pathogenesis of hyperplasia or suppression
of the erythroid hemopoietic stem [8]. Little is known
about the mechanisms of regulation of proliferation
and differentiation of erythroid precursors during experimental neuroses.Here we studied the role of the adrenergic system
and hemopoiesis-inducing microenvironment (HIM)
in the regulation of erythropoiesis during experimental
neuroses.MATERIALS AND METHODSExperiments were performed on 892 CBA/CaLac mice (class I conventional mouse strain) aging 2.0-2.5
months and obtained from the collection of the Laboratory of Experimental Biological Modeling (Institute
of Pharmacology, Tomsk Research Center).CS (10 min) [6] and PSD (48 h) [10] served as
the models of experimental neurosis. Peripheral blood
reticulocytes were counted on days 1-7 after CS and
PSD. The mice were euthanized by cervical dislocation under ether anesthesia. The count of bone marrow
erythrokaryocytes was estimated [2]. The number of
erythroid colony-forming (CFU-E) and cluster-forming units (ClFU-E) in the bone...