Content area
Full Text
Abstract [Objectives] To study the protective effects of self-made Compound Taoren Danshen Decoction (CTDD) on acute liver injury induced by D-galactosamine in rats, and to explore its mechanism. [Methods] An acute liver injury model was established by intraperitoneal injection of 500 mg/kg of D-galactosamine. The ALT, AST, MDA, SOD and GSH-Px in serum, as well as serum TNF-ct, IL-lß and IL-6 levels were measured, and liver tissue lesions were observed under microscope. [Results] CTDD can significantly reduce ALT, AST and MDA levels, increase SOD, GSH-Px activity, and significantly reduce serum TNF-ct, IL-lß and IL-6 levels, and improve liver tissue lesions. [Conclusions] CTDD has a protective effect on D-galactosamine-induced acute liver injury in rats, and its mechanism may be related to inhibition of oxidative stress and inflammatory reaction.
Key words Self-made Compound Taoren Danshen Decoction (CTDD) , D-galactosamine, Acute liver injury, Oxidative stress, Inflammatory reaction
1 Introduction
The pathogenesis of acute liver injury is complex. Studies have shown that liver injury caused by various factors is closely related to oxidative free radicals, inflammatory factors and apoptosis[11. Through summarizing the current research status of drugs for the treatment of acute liver injury at home and abroad, there is still no high effective multi-target liver-protective drug for liver injury induced by various factors. Therefore, it is of great significance to explore drugs for acute liver injury and its action mechanism. China is a large country of medicinal plant resources. It has a long history of research on traditional Chinese medicinal materials, but many valuable medicinal materials are still not effectively developed and utilized. Consisted of PERSICAE SEMEN, SALVIAE MILTIORRHIZAE RADIX ET RHIZOMA, CORTEX MOUTAN, RHODOMYRTUS TOMENTOSA, PUERA RIAE LOBATAE RADIX, and GRANGEA MADERASPATANA, the self-made CTDD has functions of promoting blood flow to eliminate stasis, benefiting dampness and stopping diarrhea, detoxifying and removing swell12 41. This experiment is intended to study the protective effects of CTDD on D-GalN-induced acute liver injury in rats, and to explore its hepatoprotective mechanism from oxidative stress and inflammatory reaction, and provide basis for the development and application of CTDD.
2 Materials and methods
2.1 Materials
2.1.1 Experimental animals. Sixty male SPF grade SD rats with weight of 180 - 220 g provided by Laboratory Animal Center of Guilin Medical University, with...