Abstract

Chromobox 6 (CBX6) is a subunit of Polycomb Repressive Complex 1 (PRC1) that mediates epigenetic gene repression and acts as an oncogene or tumor suppressor in a cancer type-dependent manner. The specific function of CBX6 in breast cancer is currently undefined. In this study, a comprehensive analysis of The Cancer Genome Atlas (TCGA) dataset led to the identification of CBX6 as a consistently downregulated gene in breast cancer. We provided evidence showing enhancer of zeste homolog 2 (EZH2) negatively regulated CBX6 expression in a Polycomb Repressive Complex 2 (PRC2)-dependent manner. Exogenous overexpression of CBX6 inhibited cell proliferation and colony formation, and induced cell cycle arrest along with suppression of migration and invasion of breast cancer cells in vitro. Microarray analyses revealed that CBX6 governs a complex gene expression program. Moreover, CBX6 induced significant downregulation of bone marrow stromal cell antigen-2 (BST2), a potential therapeutic target, via interactions with its promoter region. Our collective findings support a tumor suppressor role of CBX6 in breast cancer.

Details

Title
CBX6 is negatively regulated by EZH2 and plays a potential tumor suppressor role in breast cancer
Author
Deng Houliang 1 ; Guan Xiaowen 1 ; Gong Longcai 1 ; Zeng Jianming 1   VIAFID ORCID Logo  ; Zhang, Hongjie 1 ; Chen, Mike Y 2 ; Li, Gang 1 

1  University of Macau, Faculty of Health Sciences, Macau, China (GRID:grid.437123.0) (ISNI:0000 0004 1794 8068) 
2  City of Hope National Medical Center, Duarte, Division of Neurosurgery, Department of Surgery, California, USA (GRID:grid.410425.6) (ISNI:0000 0004 0421 8357) 
Publication year
2019
Publication date
Jan 2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-018-36560-4
ProQuest document ID
2168160422
Copyright
This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.