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Abstract
Pentoses, including D-xylose, L-arabinose, and D-arabinose, are generally phosphorylated to D-xylulose 5-phosphate in bacteria and fungi. However, in non-phosphorylative pathways analogous to the Entner-Dodoroff pathway in bacteria and archaea, such pentoses can be converted to pyruvate and glycolaldehyde (Route I) or α-ketoglutarate (Route II) via a 2-keto-3-deoxypentonate (KDP) intermediate. Putative gene clusters related to these metabolic pathways were identified on the genome of Herbaspirillum huttiense IAM 15032 using a bioinformatic analysis. The biochemical characterization of C785_RS13685, one of the components encoded to D-arabinonate dehydratase, differed from the known acid-sugar dehydratases. The biochemical characterization of the remaining components and a genetic expression analysis revealed that D- and L-KDP were converted not only to α-ketoglutarate, but also pyruvate and glycolate through the participation of dehydrogenase and hydrolase (Route III). Further analyses revealed that the Route II pathway of D-arabinose metabolism was not evolutionally related to the analogous pathway from archaea.
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1 Graduate School of Agriculture, Ehime University, Department of Bioscience, Matsuyama, Japan (GRID:grid.255464.4) (ISNI:0000 0001 1011 3808); Ehime University, Faculty of Agriculture, Matsuyama, Japan (GRID:grid.255464.4) (ISNI:0000 0001 1011 3808); Ehime University, Center for Marine Environmental Studies (CMES), Matsuyama, Japan (GRID:grid.255464.4) (ISNI:0000 0001 1011 3808)
2 Toyo University, Faculty of Food and Nutritional Sciences, Gunma, Japan (GRID:grid.265125.7) (ISNI:0000 0004 1762 8507)
3 Graduate School of Agriculture, Ehime University, Department of Bioscience, Matsuyama, Japan (GRID:grid.255464.4) (ISNI:0000 0001 1011 3808); Ehime University, Faculty of Agriculture, Matsuyama, Japan (GRID:grid.255464.4) (ISNI:0000 0001 1011 3808)
4 Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Department of Bio-molecular Engineering, Kyoto, Japan (GRID:grid.419025.b) (ISNI:0000 0001 0723 4764)