BOX-PCR fingerprinting for molecular typing of Salmonella enterica subsp. enterica serovar Enteritidis strains originated from humans, dairy cattle, and broiler chickens

Abstract

Salmonellosis is a global zoonotic infection of domestic animals and poultry with serious consequences on public health. Globally one of the most prevalent and common Salmonella enterica serotypes is serovar Enteritidis. Our goal in this research was a molecular characterization and evaluation of biodiversity and relatedness of different Salmonella isolates. Sixty-four Salmonella Enteritidis isolates which were gathered and cultured from various hosts and locations including dairy cows, broiler chicken, and patients with diarrhea of Tehran and Kerman provinces were typed by BOX-PCR assay. Dendrograms were produced by NTSYS computer software version 2.0. PCR products with different sizes from 250 to 3000 bps and the number of 5–14 bands visualized by agarose gel electrophoresis and 28 molecular patterns detected. By cluster analysis, chicken and human isolates were more similar (77.6%) than dairy cow isolates (44%). The results showed that the BOX-PCR assay is a beneficial and applicative molecular method with high discriminatory power (92.91%) for typing of the Salmonella strains and detection of heterogeneity and genetic diversity (90.52%) calculated by Shannon-Wiener index.