Abstract

Bioassay of recombinant protein products is important tests to ensure protein effectiveness. Some recombinant protein products have no cells used in their bioassay but instead use animal models, while others have no suitable method. Here, we developed a method to obtain responsive cells used in bioassay of proteins. After screening of a CRISPR/Cas9 library, we obtained a responsive cell line that grew faster in the presence of rhEGF (recombinant human epidermal growth factor) than that of control cells. We used this cell line for bioassay of rhEGF. This cell line, compared with the control cells, had a 2 day shorter operation time and had lower interference. The responsive cell line is more suitable for use in bioassay of rhEGF.

Details

Title
Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library
Author
Qin Xi 1 ; Yao Wenrong 1 ; Shi Xinchang 1 ; Liu, Lan 1 ; Huang, Fang 2 ; Ding Youxue 1 ; Zhou, Yong 1 ; Yu, Lei 1 ; Jia Chuncui 1 ; Li Shanhu 2 ; Rao, Chunming 1 ; Wang Junzhi 1 

1  National Institutes for Food and Drug Control, Department of Recombinant Products, Beijing, China (GRID:grid.410749.f) (ISNI:0000 0004 0577 6238) 
2  Beijing Institute of Biotechnology, Department of Cell Engineering, Beijing, China (GRID:grid.43555.32) (ISNI:0000 0000 8841 6246) 
Publication year
2019
Publication date
Dec 2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-019-40381-4
ProQuest document ID
2188972780
Copyright
This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.