Comparison of synthetic and natural Taxol extracted from Taxus plant (Taxus baccata) on growth of ovarian cancer cells under in vitro condition

Headnote

Abstract

At the current century, one of main challenges in human health which is costly is dealing with cancer disorders. Different approaches are applied to treat different types of cancers such as surgery, radiotherapy and chemotherapy. Recently, new technologies for drug targeting and using biosensors are developing fast to help as new solutions for cancer diseases. In this study, the anticancer potential of natural and synthetic Taxol/paclitaxel are evaluated using cell culture technique on ovarian cancer cell line (Ovcar3). Natural Taxol was extracted from Taxus baccata by two methods: classic or soaking and soxulate method. MTT assay was used for evaluation of anticancer activity of acquired extract. For this, cells were incubated with different concentration of extract. All compounds were tested in four independent experiments. The anticancer activity was recorded in three times: 24, 48, and 72 hours. According to results, the best condition for extraction of Taxol was soxulate method with ethanol as solvent. The results showed that the increase of Taxol concentration lead to higher toxicity on cancerous cells in examined three times. The comparison of activity of natural and chemical Taxol showed that there is no significant difference between two compounds at 24 and 72 hour. So, the similar observed activity for natural and chemical Taxol in this study is due to similar structures. Our study showed that the natural Taxol without any modification can use as anticancer agent

Keywords: anticancer activity, natural, synthetic, Taxol/paclitaxel, MTT, Soxhlet

Modarresi-Darreh B, Kamali K, Kalantar SM, Dehghanizadeh H, Aflatoonian B (2018) Comparison of synthetic and natural Taxol extracted from Taxus plant (Taxus baccata) on growth of ovarian cancer cells under in vitro condition. Eurasia J Biosci 12: 413-418.

© 2018 Modarresi-Darreh et al.

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INTRODUCTION

Increasing of cancer diseases is the biggest concern and a major threat for health in recent century (Abdullaev 2002, Baresari et al. 2018). Cancer is the second cause of death in the world after cardiovascular diseases which have afflicted people throughout the world. Different methods have been developed for treatment of cancer such as chemotherapy, radiotherapy, hyperthermia, and etc. (Sudhakar 2009, Taleghani et al. 2018). All these methods have adverse side effects on normal cells...