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© 2014. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Objectives: To evaluate neurotrophin (NT) (nerve growth factor [NGF], NT-3, and brain-derived neurotrophic factor [BDNF]) expression in autopsy lung tissues of human congenital diaphragmatic hernia (CDH) infants versus that of infants that expired with: 1) "normal" lungs (controls); 2) chronic lung disease (CLD); and 3) pulmonary hypertension (PPHN).

Hypothesis: NT expression will be significantly altered in CDH lung tissue compared with normal lung tissue and other neonatal lung diseases.

Study design: Immunohistochemical studies for NT proteins NGF, BDNF, and NT-3 were applied to human autopsy neonatal lung tissue samples.

Subject selection: The samples included a control group of 18 samples ranging from 23-week gestational age to term, a CDH group of 15 samples, a PPHN group of six samples, and a CLD group of 12 samples.

Methodology: The tissue samples were studied, and four representative slide fields of alveoli/saccules and four of bronchioles were recorded from each sample. These slide fields were then graded (from 0 to 3) by three blinded observers for intensity of staining.

Results: BDNF, NGF, and NT-3 immunostaining intensity scores were significantly decreased in the CDH lung tissue (n=15) compared with normal neonatal lung tissue (n=18) (P<0.001). The other neonatal pulmonary diseases that were studied, CLD and PPHN, were much less likely to be affected and were much more variable in their neurotrophin expression.

Conclusion: NT expression is decreased in CDH lungs. The decreased expression of NT in CDH lung tissue may suggest they contribute to the abnormality in this condition.

Details

Title
Neurotrophins expression is decreased in lungs of human infants with congenital diaphragmatic hernia
Author
Lynn D O’Hanlon; Mabry, Sherry M; Ekekezie, Ikechukwu I
Pages
9-15
Section
Original Research
Publication year
2014
Publication date
2014
Publisher
Taylor & Francis Ltd.
ISSN
1179-9935
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2228708254
Copyright
© 2014. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.