Content area

Abstract

We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells. [PUBLICATION ABSTRACT]

Details

Title
Using buoyant mass to measure the growth of single cells
Author
Godin, Michel; Delgado, Francisco Feijó; Son, Sungmin; Grover, William H; Bryan, Andrea K; Tzur, Amit; Jorgensen, Paul; Payer, Kris; Grossman, Alan D; Kirschner, Marc W; Manalis, Scott R
Pages
387-90
Publication year
2010
Publication date
May 2010
Publisher
Nature Publishing Group
ISSN
15487091
e-ISSN
15487105
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/nmeth.1452
ProQuest document ID
223230795
Copyright
Copyright Nature Publishing Group May 2010