Content area
Abstract
Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells. [PUBLICATION ABSTRACT]
Details
Title
Lifeact: a versatile marker to visualize F-actin
Author
Riedl, Julia; Crevenna, Alvaro H; Kessenbrock, Kai; Yu, Jerry Haochen; Neukirchen, Dorothee; Bista, Michal; Bradke, Frank; Jenne, Dieter; Holak, Tad A; Werb, Zena; Sixt, Michael; Wedlich-soldner, Roland
Pages
605-7
Publication year
2008
Publication date
Jul 2008
ISSN
15487091
e-ISSN
15487105
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
223248614
Copyright
Copyright Nature Publishing Group Jul 2008