Abstract

Folded single chain polymeric nano-objects are the molecular level soft material with ultra-small size. Here, we report an easy and scalable method for preparing single-chain nanogels (SCNGs) with improved efficiency. We further investigate the impact of the dynamic molecular conformational change of SCNGs on cellular interactions from molecular to bulk scale. First, the supramolecular unfoldable SCNGs efficiently deliver siRNAs into stem cells as a molecular drug carrier in a conformation-dependent manner. Furthermore, the conformation changes of SCNGs enable dynamic and precise manipulation of ligand tether structure on 2D biomaterial interfaces to regulate the ligand–receptor ligation and mechanosensing of cells. Lastly, the dynamic SCNGs as the building blocks provide effective energy dissipation to bulk biomaterials such as hydrogels, thereby protecting the encapsulated stem cells from deleterious mechanical shocks in 3D matrix. Such a bottom-up molecular tailoring strategy will inspire further applications of single-chain nano-objects in the biomedical area.

Details

Title
Conformational manipulation of scale-up prepared single-chain polymeric nanogels for multiscale regulation of cells
Author
Chen, Xiaoyu 1 ; Li, Rui 1 ; Siu Hong Dexter Wong 1   VIAFID ORCID Logo  ; Kongchang Wei 2   VIAFID ORCID Logo  ; Cui, Miao 3 ; Chen, Huaijun 4 ; Jiang, Yuanzhang 5 ; Yang, Boguang 1 ; Zhao, Pengchao 1 ; Xu, Jianbin 6 ; Chen, Heng 7 ; Yin, Chao 1 ; Lin, Sien 8 ; Lee, Wayne Yuk-Wai 8 ; Yihan Jing 1 ; Li, Zhen 4 ; Yang, Zhengmeng 8 ; Jiang, Xia 9 ; Chen, Guosong 4   VIAFID ORCID Logo  ; Li, Gang 8 ; Bian, Liming 10   VIAFID ORCID Logo 

1  Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, Hong Kong 
2  Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomimetic Membranes and Textiles, St. Gallen, Switzerland 
3  Beijing Genomic Institute-Shenzhen, Shenzhen, China 
4  The State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, China 
5  Institute of Textiles & Clothing, The Hong Kong Polytechnic University, Hong Kong, Hong Kong 
6  Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China 
7  Shenzhen Key Laboratory of Special Functional Materials, College of Materials Science and Engineering, Shenzhen University, Shenzhen, China 
8  Department of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, Hong Kong; The CUHK-ACC Space Medicine Centre on Health Maintenance of Musculoskeletal System, Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China 
9  Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, Hong Kong 
10  Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, Hong Kong; Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China; Centre for Novel Biomaterials, The Chinese University of Hong Kong, Hong Kong, Hong Kong; China Orthopaedic Regenerative Medicine Group, Hangzhou, China 
Pages
1-12
Publication year
2019
Publication date
Jun 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-019-10640-z
ProQuest document ID
2244136689
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.