Abstract

Brassica napus, an allotetraploid crop, is hypothesized to be a hybrid from unknown varieties of Brassica rapa and Brassica oleracea. Despite the economic importance of B. napus, much is unresolved regarding its phylogenomic relationships, genetic structure, and diversification. Here we conduct a comprehensive study among diverse accessions from 183 B. napus (including rapeseed, rutabaga, and Siberian kale), 112 B. rapa, and 62 B. oleracea and its wild relatives. Using RNA-seq of B. napus accessions, we define the genetic diversity and sub-genome variance of six genetic clusters. Nuclear and organellar phylogenies for B. napus and its progenitors reveal varying patterns of inheritance and post-formation introgression. We discern regions with signatures of selective sweeps and detect 8,187 differentially expressed genes with implications for B. napus diversification. This study highlights the complex origin and evolution of B. napus providing insights that can further facilitate B. napus breeding and germplasm preservation.

Details

Title
Transcriptome and organellar sequencing highlights the complex origin and diversification of allotetraploid Brassica napus
Author
An, Hong 1   VIAFID ORCID Logo  ; Xinshuai Qi 2 ; Gaynor, Michelle L 3   VIAFID ORCID Logo  ; Yue Hao 4   VIAFID ORCID Logo  ; Gebken, Sarah C 5 ; Mabry, Makenzie E 5 ; McAlvay, Alex C 6 ; Teakle, Graham R 7 ; Conant, Gavin C 4 ; Barker, Michael S 2   VIAFID ORCID Logo  ; Fu, Tingdong 8 ; Yi, Bin 8 ; Pires, J Chris 5   VIAFID ORCID Logo 

1  Division of Biological Sciences, University of Missouri, Columbia, MO, USA; National Key Lab of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, P. R. China 
2  Department of Ecology & Evolutionary Biology, University of Arizona, Tucson, AZ, USA 
3  Department of Biology, University of Central Florida, Orlando, FL, USA 
4  Bioinformatics Research Center, North Carolina State University, Raleigh, NC, USA 
5  Division of Biological Sciences, University of Missouri, Columbia, MO, USA 
6  Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY, USA 
7  School of Life Sciences, University of Warwick, Coventry, UK 
8  National Key Lab of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, P. R. China 
Pages
1-12
Publication year
2019
Publication date
Jun 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-019-10757-1
ProQuest document ID
2249019783
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.