Abstract

Gossypol is a toxic polyphenolic product that is derived from cotton plants. The toxicity of gossypol has limited the utilization of cottonseed meal (CSM) in the feed industry. The gene, Helicoverpa armigera CYP9A12, is a gossypol-inducible cytochrome P450 gene. The objective of our study was to obtain the functional recombinant H. armigera CYP9A12 enzyme in Pichia pastoris and to verify whether this candidate enzyme could decrease gossypol in vitro. Free and total gossypol contents were detected in the enzyme solution and in CSM. The H. armigera CYP9A12 enzyme degraded free concentration of gossypol. After optimization of the single-test and response surface method, free gossypol content could be decreased to 40.91 mg/kg in CSM by the H. armigera CYP9A12 enzyme when the initial temperature was 35 °C, the enzymatic hydrolysis time lasted 2.5 h, the enzyme addition was 2.5 mL, and the substrate moisture was 39%.

Details

Title
Optimization of the process parameters for reduction of gossypol levels in cottonseed meal by functional recombinant NADPH-cytochrome P450 reductase and cytochrome P450 CYP9A12 of Helicoverpa armigera
Author
Chen, Cheng 1 ; Zhang, Yan 2 ; Pi, Wenhui 3 ; Yang, Wenting 1 ; Nie, Cunxi 4 ; Liang, Jing 1 ; Ma, Xi 4 ; Wen-ju, Zhang 1   VIAFID ORCID Logo 

1  College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China 
2  School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, Xinjiang, China 
3  State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, Xinjiang, China 
4  College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China; State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China 
Pages
1-10
Publication year
2019
Publication date
Jul 2019
Publisher
Springer Nature B.V.
e-ISSN
21910855
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s13568-019-0823-4
ProQuest document ID
2252642960
Copyright
AMB Express is a copyright of Springer, (2019). All Rights Reserved., © 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.