Abstract

Dual-emissive systems showing color-specific photoswitching are promising in bioimaging and super-resolution microscopy. However, their switching efficiency has been limited because a delicate manipulation of all the energy transfer crosstalks in the systems is unfeasible. Here, we report a perfect color-specific photoswitching, which is rationally designed by combining the complete off-to-on fluorescence switching capability of a fluorescent photochromic diarylethene and the frustrated energy transfer to the other fluorescent dye based on the excited-state intramolecular proton transfer (ESIPT) process. Upon alternation of UV and visible light irradiations, the system achieves 100% switching on/off of blue emission from the diarylethene while orange emission from the ESIPT dye is unchanged in the polymer film. By fabricating this system into biocompatible polymer nanoparticles, we demonstrate microscopic imaging of RAW264.7 macrophage cells with reversible blue-color specific fluorescence switching that enables super-resolution imaging with a resolution of 70 nm.

Details

Title
Dual-color fluorescent nanoparticles showing perfect color-specific photoswitching for bioimaging and super-resolution microscopy
Author
Kim, Dojin 1   VIAFID ORCID Logo  ; Jeong, Keunsoo 2   VIAFID ORCID Logo  ; Kwon, Ji Eon 1   VIAFID ORCID Logo  ; Park, Hyeonjong 2 ; Lee, Seokyung 2 ; Kim, Sehoon 3   VIAFID ORCID Logo  ; Soo Young Park 1 

1  Center for Supramolecular Optoelectronic Materials, Research Institute of Advanced Materials (RIAM), Department of Materials Science and Engineering, Seoul National University, Gwanak-gu, Seoul, Korea 
2  Center for Theragnosis, Korea Institute of Science and Technology, Seongbuk-gu, Seoul, Korea 
3  Center for Theragnosis, Korea Institute of Science and Technology, Seongbuk-gu, Seoul, Korea; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seongbuk-gu, Seoul, Korea 
Pages
1-10
Publication year
2019
Publication date
Jul 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-019-10986-4
ProQuest document ID
2256665603
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.