Abstract

While genomics links gene activity with disease, proteomics investigates gene expression i.e. the proteins which are the ultimate effector molecules; and hence more relevant to identification of biomarkers. The basic steps of proteomics include (i) albumin removal; (ii) proteins digestion; (iii) protein separation step; and (iv) analysis by mass spectrometry [2],[5],[6],[7]. The methods commonly used currently for proteomic analysis are [6] : (i) two-dimensional gel electrophoresis followed by mass spectrometry (2DE-MS); (ii) liquid chromatography followed by mass spectrometry (LC-MS).

Details

Title
Serum proteomics for the diagnosis of nephrotic syndrome: Is there a ray of hope?
Author
Bhowmik, Dipankar 1 ; Agarwal, Sanjay 1 

 Department of Nephrology, All India Institute of Medical Sciences, New Delhi 110 029 
Pages
273-275
Publication year
2012
Publication date
Mar 2012
Publisher
Scientific Scholar
ISSN
0971-5916
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2258259818
Copyright
© 2012. This work is published under https://creativecommons.org/licenses/by-nc-sa/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.