Content area
Abstract
Re-domestication of potato into an inbred line-based diploid crop propagated by seed represents a promising alternative to traditional clonal propagation of tetraploid potato, but self-incompatibility has hindered the development of inbred lines. To address this problem, we created self-compatible diploid potatoes by knocking out the self-incompatibility gene S-RNase using the CRISPR–Cas9 system. This strategy opens new avenues for diploid potato breeding and will also be useful for studying other self-incompatible crops.
Details
Title
Generation of self-compatible diploid potato by knockout of S-RNase
Author
Ye, Mingwang 1 ; Peng, Zhen 1 ; Tang, Die 2 ; Yang, Zhongmin 3 ; Li, Dawei 3 ; Xu, Yunmei 4 ; Zhang, Chunzhi 5 ; Huang, Sanwen 5
1 The CAAS-YNNU Joint Academy of Potato Sciences, Yunnan Normal University, Kunming, China; Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
2 Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
3 College of Horticulture, Northwest Agriculture and Forest University, Yangling, China
4 The CAAS-YNNU Joint Academy of Potato Sciences, Yunnan Normal University, Kunming, China
5 Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China
1 The CAAS-YNNU Joint Academy of Potato Sciences, Yunnan Normal University, Kunming, China; Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
2 Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
3 College of Horticulture, Northwest Agriculture and Forest University, Yangling, China
4 The CAAS-YNNU Joint Academy of Potato Sciences, Yunnan Normal University, Kunming, China
5 Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China
Pages
651-654
Publication year
2018
Publication date
Sep 2018
e-ISSN
20550278
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2272203390
Copyright
Copyright Nature Publishing Group Sep 2018