Abstract

In this study, we isolated a total of 238 culturable putative bacterial endophytes from four Pinus species (Pinus densiflora, P. koraiensis, P. rigida, and P. thunbergii) across 18 sampling sites in Korea. The samples were cultured in de Man Rogosa Sharpe and humic acid-vitamin agar media. These selective media were used to isolate lactic acid bacteria and Actinobacteria, respectively. Analysis using 16S ribosomal DNA sequencing grouped the isolated putative bacterial endophytes into 107 operational taxonomic units (OTUs) belonging to 48 genera. Gamma-proteobacteria were the most abundant bacteria in each sampling site and three tissues (needle, stem and root). The highest OTU richness and diversity indices were observed in the roots, followed by stem and needle tissues. Total metabolites extracted from three isolates (two isolates of Escherichia coli and Serratia marcescens) showed significant nematicidal activity against the pine wood nematode (Bursaphelenchus xylophilus). Our findings demonstrated the potential use of bacterial endophytes from pine trees as alternative biocontrol agents against pine wood nematodes.

Details

Title
Distribution and diversity of bacterial endophytes from four Pinus species and their efficacy as biocontrol agents for devastating pine wood nematodes
Author
Liu, Yunran 1 ; Lakshmi Narayanan Ponpandian 1 ; Hoki, Kim 1 ; Jeon, Junhyun 1 ; Buyng Su Hwang 2   VIAFID ORCID Logo  ; Lee, Sun Keun 3 ; Soo-Chul Park 4 ; Bae, Hanhong 1 

1  Department of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, Republic of Korea 
2  Nakdonggang National Institute of Biological Resources, Sangju, Gyeongbuk, Republic of Korea 
3  Division of Forest Insect Pests and Diseases, National Institute of Forest Science, Seoul, Republic of Korea 
4  Crop Biotechnology Institute, Green Bio Science & Technology, Seoul National University, Pyeongchang, Kangwon, Republic of Korea 
Pages
1-12
Publication year
2019
Publication date
Aug 2019
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-019-48739-4
ProQuest document ID
2281973147
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.