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© 2016. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Premise of the study:

We designed and tested microsatellite markers for the North American native species Silene stellata (Caryophyllaceae) to investigate its population genetic structure and identify selection on floral design through male reproductive success.

Methods and Results:

A total of 153 candidate microsatellite loci were isolated based on next‐generation sequencing. We identified 18 polymorphic microsatellite loci in three populations of S. stellata, with di‐ or trinucleotide repeats. Genotyping results showed the number of alleles per locus ranged from six to 45 and expected heterozygosity ranged from 0.511 to 0.951. Five of these loci were successfully amplified in S. virginica and S. caroliniana and were also polymorphic.

Conclusions:

The microsatellite markers reported here provide a valuable tool for paternity analysis in S. stellata. They will also be useful for investigating the population genetic structures of S. stellata and related species.

Details

Title
Development of highly variable microsatellite markers for the tetraploid Silene stellata (Caryophyllaceae)
Author
Zhou, Juannan 1 ; Dudash, Michele R 2 ; Fenster, Charles B 3 ; Zimmer, Elizabeth A 4 

 Behavior, Ecology, Evolution and Systematics Graduate Program, University of Maryland, College Park, Maryland 20742 USA; Department of Botany, National Museum of Natural History, MRC 166, Smithsonian Institution, Washington, DC 20013‐7012 USA 
 Department of Natural Resource Management, South Dakota State University, Brookings, South Dakota 57007 USA 
 Department of Biology and Microbiology, South Dakota State University, Brookings, South Dakota 57007 USA 
 Department of Botany, National Museum of Natural History, MRC 166, Smithsonian Institution, Washington, DC 20013‐7012 USA 
Section
Primer Notes
Publication year
2016
Publication date
Dec 2016
Publisher
John Wiley & Sons, Inc.
e-ISSN
21680450
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2288639366
Copyright
© 2016. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.