Abstract

This study used a two-stage culture system to efficiently produce natural killer (NK) cells from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in the absence of cell sorting and without need for xenogeneic stromal cells. Although different hESC and iPSC lines had varying efficiencies in hematopoietic development, all cell lines tested could produce functional NK cells. This improved method to develop NK cells from human pluripotent stem cells provides a system for clinical-scale expansion of antitumor lymphocytes and a genetically amenable platform to study human NK cell development.

Details

Title
Clinical-Scale Derivation of Natural Killer Cells From Human Pluripotent Stem Cells for Cancer Therapy
Author
Knorr, David A 1 ; Ni Zhenya 1 ; Hermanson, David 1 ; Hexum, Melinda K 1 ; Bendzick Laura 1 ; Cooper Laurence J.N. 2 ; Lee, Dean A 2 ; Kaufman, Dan S 3 

 Department of Medicine (Hematology, Oncology, and Transplant); Stem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA 
 Division of Pediatrics, M.D. Anderson Cancer Center; School of Biomedical Sciences, Health Science Center, University of Texas, Houston, Texas, USA 
 Department of Medicine (Hematology, Oncology, and Transplant); Stem Cell Institute, University of Minnesota, Minneapolis, Minnesota, USA; International Clinical Research Center, Integrated Center of Cellular Therapy and Regenerative Medicine, St. Anne's University Hospital Brno, Brno, Czech Republic 
Pages
274-283
Section
Protocols and Manufacturing for Cell-Based Therapies
Publication year
2013
Publication date
Apr 2013
Publisher
Oxford University Press
ISSN
21576564
e-ISSN
21576580
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2290073607
Copyright
© 2013. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.