The Use of Hepatic and Somatic Indices and Histological Information to Characterize the Reproductive Dynamics of Atlantic Sardine Sardina pilchardus from the Portuguese Coast

Abstract

Samples of Atlantic sardine Sardina pilchardus (also known as European pilchard) were collected bimonthly from 2004 to 2008 off the central west coast of Portugal to describe the reproductive activity of this indeterminate batch‐spawning species; compare the seasonality of somatic growth, condition, and feeding; and evaluate differences between sexes. Monthly assessments of individual biological information for both males and females were complemented by histological analysis of ovaries during 1 year and liver tissues (both sexes) at different times of the reproductive cycle. The temporal patterns of the gonadosomatic index and various histological indices (most advanced oocyte stage, atresia incidence and prevalence, and spawning activity) indicated that Atlantic sardine were reproductively active mainly from October to March and that residual activity occurred in the remaining months. For both sexes, condition indices (hepatosomatic index, relative weight, and amount of fat stored) increased mainly during spring, reached a maximum at the end of summer just before the subsequent spawning season began, and then decreased during autumn and winter, declining to minimum levels at the beginning of spring coincident with a significant reduction in reproductive activity. Somatic growth took place mainly during spring and early summer for both sexes. The observed seasonal patterns in these biological properties suggest a seasonal transition from a period in which energy resources are allocated to reproduction (autumn and winter) to a period in which resources are allocated to growth and fat deposition (spring and summer). The only exception was the distinct hepatosomatic index pattern and the histological differences in hepatocytes between males and females during the spawning season, which may be related to the dual function of the liver in females (lipid metabolism and yolk precursor synthesis).