Abstract

This study reports the successful reprogramming of human fibroblasts into a state of pluripotency by baculoviral transduction-mediated, site-specific integration of OKSM transcription factor genes into the AAVS1 locus in human chromosome 19. Methods based on site-specific integration of reprogramming factor genes as reported here hold the potential for efficient generation of genetically amenable induced pluripotent stem cells suitable for future gene therapy applications.

Details

Title
Zinc Finger Nuclease-Expressing Baculoviral Vectors Mediate Targeted Genome Integration of Reprogramming Factor Genes to Facilitate the Generation of Human Induced Pluripotent Stem Cells
Author
Rui-Zhe, Phang 1 ; Tay Felix Chang 1 ; Sal-Lee, Goh 1 ; Lau Cia-Hin 1 ; Zhu Haibao 1 ; Wee-Kiat, Tan 1 ; Liang Qingle 1 ; Chen, Can 1 ; Du Shouhui 1 ; Li, Zhendong 1 ; Chin-Kang, Tay Johan 1 ; Wu, Chunxiao 2 ; Zeng Jieming 2 ; Fan Weimin 3 ; Toh Han Chong 4 ; Wang, Shu 5 

1  Department of Biological Sciences, National University of Singapore, Singapore 
2  Institute of Bioengineering and Nanotechnology, Singapore 
3  Program of Innovative Cancer Therapeutics, Department of Surgery, First Affiliated Hospital of Zhejiang University College of Medicine, Hangzhou, China 
4  National Cancer Centre Singapore, Singapore 
5  Department of Biological Sciences, National University of Singapore, Singapore; Institute of Bioengineering and Nanotechnology, Singapore 
Pages
935-945
Section
Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells
Publication year
2013
Publication date
Dec 2013
Publisher
Oxford University Press
ISSN
21576564
e-ISSN
21576580
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.5966/sctm.2013-0043
ProQuest document ID
2290256979
Copyright
© 2013. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.