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Abstract
High-resolution imaging techniques were used to analyze the relationship between a Wnt-responsive cancer stem cell (CSC)-enriched population and the tumor vasculature using p53-null mouse mammary tumors transduced with a Wnt signaling pathway reporter. The results demonstrate that the combined strategy of monitoring the fluorescently labeled CSCs and vasculature using high-resolution imaging techniques provides a unique opportunity to study the CSC microenvironment.
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1 Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, USA
2 Lester & Sue Smith Breast Center, Departments of Molecular and Cellular Biology and Radiology, Baylor College of Medicine, Houston, Texas, USA; Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA
3 Department of Systems Medicine and Bioengineering, The Methodist Hospital Research Institute, Weill Cornell Medical College, Houston, Texas, USA
4 Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA; Department of Systems Medicine and Bioengineering, The Methodist Hospital Research Institute, Weill Cornell Medical College, Houston, Texas, USA
5 Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA
6 Department of Developmental Biology, Pittsburgh, Pennsylvania, USA; Women's Cancer Research Center, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania, USA