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© 2014. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

In its early years, mass spectrometry (MS)-based proteomics focused on the cataloging of proteins found in different species or different tissues. By 2005, proteomics was being used for protein quantitation, typically based on “proteotypic” peptides which act as surrogates for the parent proteins. Biomarker discovery is usually done by non-targeted “shotgun” proteomics, using relative quantitation methods to determine protein expression changes that correlate with disease (output given as “up-or-down regulation” or “fold-increases”). MS-based techniques can also perform “absolute” quantitation which is required for clinical applications (output given as protein concentrations). Here we describe the differences between these methods, factors that affect the precision and accuracy of the results, and some examples of recent studies using MS-based proteomics to verify cancer-related biomarkers.

Details

Title
Mass spectrometry based biomarker discovery, verification, and validation — Quality assurance and control of protein biomarker assays
Author
Parker, Carol E 1 ; Borchers, Christoph H 2 

 University of Victoria – Genome British Columbia Proteomics Centre, Vancouver Island Technology Park, #3101 – 4464 Markham St., Victoria, BC V8Z 7X8, Canada 
 University of Victoria – Genome British Columbia Proteomics Centre, Vancouver Island Technology Park, #3101 – 4464 Markham St., Victoria, BC V8Z 7X8, Canada; Department of Biochemistry and Microbiology, University of Victoria, Petch Building Room 207, 3800 Finnerty Rd., Victoria, BC V8P 5C2, Canada 
Pages
840-858
Section
Reviews
Publication year
2014
Publication date
Jun 2014
Publisher
John Wiley & Sons, Inc.
ISSN
15747891
e-ISSN
18780261
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2299145753
Copyright
© 2014. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.