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© 2014. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Several biomarkers have been proposed as useful parameters to better specify the prognosis or to delineate new target therapy strategies for glioblastoma patients. MicroRNAs could represent putative target molecules, considering their role in tumorigenesis, cancer progression and their specific tissue expression. Although several studies have tried to identify microRNA signature for glioblastoma, a microRNA profile is still far from being well-defined.

In this work the expression of 19 microRNAs (miR-7, miR-9, miR-9∗, miR-10a, miR-10b, miR-17, miR-20a, miR-21, miR-26a, miR-27a, miR-31, miR-34a, miR-101, miR-137, miR-182, miR-221, miR-222, miR-330, miR-519d) was evaluated in sixty formalin-fixed and paraffin-embedded glioblastoma samples using a locked nucleic acid real-time PCR. Moreover, a comparison of miRNA expressions was performed between primary brain neoplasias of different grades (grades IV–I).

The analysis of 14 validated miRNA expression in the 60 glioblastomas, using three different non-neoplastic references as controls, revealed a putative miRNA signature: mir-10b and miR-21 were up-regulated, while miR-7, miR-31, miR-101, miR-137, miR-222 and miR-330 were down-regulated in glioblastomas. Comparing miRNA expression between glioblastoma group and gliomas of grades I–III, 3 miRNAs (miR-10b, mir-34a and miR-101) showed different regulation statuses between high-grade and low-grade tumors. miR-10b was up-regulated in high grade and significantly down-regulated in low-grade gliomas, suggesting that could be a candidate for a GBM target therapy.

This study provides further data for the identification of a miRNA profile for glioblastoma and suggests that different-grade neoplasia could be characterized by different expression of specific miRNAs.

Details

Title
Expression of 19 microRNAs in glioblastoma and comparison with other brain neoplasia of grades I–III
Author
Visani, Michela 1 ; de Biase, Dario 2 ; Marucci, Gianluca 3 ; Cerasoli, Serenella 4 ; Nigrisoli, Evandro 4 ; Maria Letizia Bacchi Reggiani 5 ; Albani, Fiorenzo 6 ; Baruzzi, Agostino 6 ; Pession, Annalisa 1 

 Department of Pharmacy and Biotechnology, FaBiT, University of Bologna, via Belmeloro 6, 40126 Bologna, Italy 
 Department of Pharmacy and Biotechnology, FaBiT, University of Bologna, via Belmeloro 6, 40126 Bologna, Italy; Department of Experimental, Diagnostic and Specialty Medicine, DIMES, Anatomic Pathology at Bellaria Hospital, University of Bologna, via Altura 3, 40139 Bologna, Italy 
 Department of Biomedical Science and Neuromotor Sciences, DIBINEM, Anatomic Pathology, Bellaria Hospital, University of Bologna, via Altura 3, 40139 Bologna, Italy 
 Anatomic Pathology, M. Bufalini Hospital, Viale Ghirotti 286, 47023 Cesena (FC), Italy 
 Department of Experimental, Diagnostic and Specialty Medicine, DIMES, University of Bologna, Via Massarenti 9, 40100 Bologna, Italy 
 IRCCS Istituto delle Scienze Neurologiche, via Altura 3, 40139 Bologna, Italy; Department of Biomedical & Neuromotor Sciences, University of Bologna, via Altura 3, 40139 Bologna, Italy 
Pages
417-430
Section
Research Articles
Publication year
2014
Publication date
Mar 2014
Publisher
John Wiley & Sons, Inc.
ISSN
15747891
e-ISSN
18780261
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2299178708
Copyright
© 2014. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.