Abstract

Deinococcus radiodurans shows extreme resistance to a range of remarkable environmental stresses. Deinococcal exopolysaccharide (DeinoPol) is a component of the cell wall, but its role in stress resistance has not yet been well-described. In this study, we isolated and characterized DeinoPol from Deinococcus radiodurans R1 strain and investigated its application as an antioxidant agent. Bioinformatic analysis indicated that dra0033, encoding an ExoP-like protein, was involved in DeinoPol biosynthesis, and dra0033 mutation significantly decreased survival rates in response to stresses. Purified DeinoPol consists of different monosaccharides and has a molecular weight of approximately 80 to 100 kDa. DeinoPol also demonstrates highly protective effects on human keratinocytes in response to stress-induced apoptosis by effectively scavenging ROS. Taken together, these findings indicate that DeinoPol is the first reported deinococcal exopolysaccharide that might be used in cosmetics and pharmaceuticals as a safe and attractive radical scavenger.

Details

Title
Antioxidant Activities of an Exopolysaccharide (DeinoPol) Produced by the Extreme Radiation-Resistant Bacterium Deinococcus radiodurans
Author
Lin, Shun Mei 1   VIAFID ORCID Logo  ; Baek Chan Yu 2 ; Jong-Hyun, Jung 3 ; Kim, Woo Sik 4 ; Ha-Yeon, Song 4 ; Lee, Ji Hee 4 ; Jung, Ji Hyun 4 ; Yong, Zhi 3 ; Kang Bo Sun 5 ; Yong-Sun, Bahn 6 ; Seo Ho Seong 3 ; Lim Sangyong 3   VIAFID ORCID Logo 

1  Korea Atomic Energy Research Institute, Research Division for Radiation Science, Jeongeup, Republic of Korea (GRID:grid.418964.6) (ISNI:0000 0001 0742 3338) ; R&D Center, MediNResearch, Jeongeup, Republic of Korea (GRID:grid.418964.6) 
2  Korea Atomic Energy Research Institute, Research Division for Radiation Science, Jeongeup, Republic of Korea (GRID:grid.418964.6) (ISNI:0000 0001 0742 3338) ; R&D Center, MediNResearch, Jeongeup, Republic of Korea (GRID:grid.418964.6) ; Yonsei University, Department of Biotechnology, College of Life Science and Biotechnology, Seoul, Republic of Korea (GRID:grid.15444.30) (ISNI:0000 0004 0470 5454) 
3  Korea Atomic Energy Research Institute, Research Division for Radiation Science, Jeongeup, Republic of Korea (GRID:grid.418964.6) (ISNI:0000 0001 0742 3338) ; University of Science and Technology, Department of Radiation Science, Daejeon, Republic of Korea (GRID:grid.412786.e) (ISNI:0000 0004 1791 8264) 
4  Korea Atomic Energy Research Institute, Research Division for Radiation Science, Jeongeup, Republic of Korea (GRID:grid.418964.6) (ISNI:0000 0001 0742 3338) 
5  Konyang University, Department of Radiological Science, Daejeon, Republic of Korea (GRID:grid.411143.2) (ISNI:0000 0000 8674 9741) 
6  Yonsei University, Department of Biotechnology, College of Life Science and Biotechnology, Seoul, Republic of Korea (GRID:grid.15444.30) (ISNI:0000 0004 0470 5454) 
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-019-56141-3
ProQuest document ID
2342990738
Copyright
This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.