Abstract

The rhesus macaque is a prime model animal in neuroscience. A comprehensive transcriptomic and open chromatin atlas of the rhesus macaque brain is key to a deeper understanding of the brain. Here we characterize the transcriptome of 416 brain samples from 52 regions of 8 rhesus macaque brains. We identify gene modules associated with specific brain regions like the cerebral cortex, pituitary, and thalamus. In addition, we discover 9703 novel intergenic transcripts, including 1701 coding transcripts and 2845 lncRNAs. Most of the novel transcripts are only expressed in specific brain regions or cortical regions of specific individuals. We further survey the open chromatin regions in the hippocampal CA1 and several cerebral cortical regions of the rhesus macaque brain using ATAC-seq, revealing CA1- and cortex-specific open chromatin regions. Our results add to the growing body of knowledge regarding the baseline transcriptomic and open chromatin profiles in the brain of the rhesus macaque.

Non-human primates share many features with humans and are an important animal model in neuroscience. Here, the authors present a comprehensive transcriptomic and open chromatin atlas of the rhesus macaque brain.

Details

Title
Transcriptomic and open chromatin atlas of high-resolution anatomical regions in the rhesus macaque brain
Author
Yin Senlin 1 ; Lu Keying 1 ; Tan, Tao 2 ; Tang, Jie 1   VIAFID ORCID Logo  ; Jingkuan, Wei 3 ; Liu, Xu 4 ; Hu Xinlei 1 ; Wan Haisu 4 ; Huang, Wei 5 ; Fan, Yong 6 ; Xie, Dan 1   VIAFID ORCID Logo  ; Yang, Yu 7   VIAFID ORCID Logo 

1  Sichuan University, Frontier Science Center for Disease Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Chengdu, China (GRID:grid.13291.38) (ISNI:0000 0001 0807 1581) 
2  Peking University Third Hospital, Department of Obstetrics and Gynecology, Beijing, China (GRID:grid.411642.4) (ISNI:0000 0004 0605 3760); Kunming University of Science and Technology, Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming, China (GRID:grid.218292.2) (ISNI:0000 0000 8571 108X); The Third Affiliated Hospital of Guangzhou Medical University, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou, China (GRID:grid.417009.b) (ISNI:0000 0004 1758 4591) 
3  Kunming University of Science and Technology, Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming, China (GRID:grid.218292.2) (ISNI:0000 0000 8571 108X) 
4  The Affiliated Hospital of Southwest Medical University, Experiment Medicine Center, Luzhou, China (GRID:grid.488387.8) 
5  Northeast Normal University, School of Mathematics and Statistics, Key Laboratory for Applied Statistics of the Ministry of Education, Changchun, China (GRID:grid.27446.33) (ISNI:0000 0004 1789 9163) 
6  The Third Affiliated Hospital of Guangzhou Medical University, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou, China (GRID:grid.417009.b) (ISNI:0000 0004 1758 4591) 
7  Peking University Third Hospital, Department of Obstetrics and Gynecology, Beijing, China (GRID:grid.411642.4) (ISNI:0000 0004 0605 3760) 
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-020-14368-z
ProQuest document ID
2344544554
Copyright
This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.