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Abstract
Correlating data from different microscopy techniques holds the potential to discover new facets of signaling events in cellular biology. Here we report for the first time a hardware set-up capable of achieving simultaneous co-localized imaging of spatially correlated far-field super-resolution fluorescence microscopy and atomic force microscopy, a feat only obtained until now by fluorescence microscopy set-ups with spatial resolution restricted by the Abbe diffraction limit. We detail system integration and demonstrate system performance using sub-resolution fluorescent beads and applied to a test sample consisting of human bone osteosarcoma epithelial cells, with plasma membrane transporter 1 (MCT1) tagged with an enhanced green fluorescent protein (EGFP) at the N-terminal.
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Details
1 International Iberian Nanotechnology Laboratory, Braga, Portugal (GRID:grid.420330.6) (ISNI:0000 0004 0521 6935); University of Santiago de Compostela, Department of Applied Physics, Santiago de Compostela, Spain (GRID:grid.11794.3a) (ISNI:0000000109410645)
2 JPK BioAFM Business, Nano Surfaces Division, Bruker Nano GmbH, Berlin, Germany (GRID:grid.11794.3a)
3 International Iberian Nanotechnology Laboratory, Braga, Portugal (GRID:grid.420330.6) (ISNI:0000 0004 0521 6935)
4 University of Minho, Centre of Molecular and Environmental Biology, Department of Biology, Braga, Portugal (GRID:grid.10328.38) (ISNI:0000 0001 2159 175X)
5 University of California, Berkeley, Department of Molecular and Cell Biology, Berkeley, USA (GRID:grid.47840.3f) (ISNI:0000 0001 2181 7878)