Abstract

Context: Andrographolide (Andro), found in large quantities in Andrographis paniculata Nees (Acanthaceae), is anti-inflammatory, especially in the central nervous system (CNS) glia.

Objective: The objective of this study is to test Andro’s ability to reduce allodynia in a spared nerve injury model.

Material and methods: Male 30 g BalbC mice were divided into four groups: (1) Sham-operated control (Sham-group); (2) nerve injured and treated with saline (Saline-group); (3) nerve injured and treated with Andro (Andro-group); (4) nerve injured and treated with non-steroidal anti-inflammatory drugs (NSAIDS) (NSAIDS-group). Andro or NSAIDS (diclofenac salt) were injected intraperitoneally at 5 mg/kg body weight daily. Mechanical allodynia was assessed by von Frey tests at 3, 7, and 14 d. For immunohistochemical analysis, samples were collected at 7 d.

Results: The threshold for inducing allodynia increased and the response percentage reduced in the Andro-group when compared with the Saline-group, as well as when compared with NSAIDS groups throughout 3–14 d. The ratio of threshold for OP-Andro/OP-saline and for OP-Andro/OP-NSAIDS groups was 20.42 and 11.67 at 14 d, respectively. The ratio of response percentage for OP-Andro/OP-saline and for OP-Andro/OP-NSAIDS was 0.32 and 0.39 at 14 d, respectively. Interleukin-1 (IL-1) immunostaining in the spinal cord was reduced in the Andro-group. Astrocytic activities were not significantly reduced in the Andro-group compared with the Saline-group at 7 d post-operation (PO)

Conclusions: Andro reduced mechanical allodynia more than NSAIDS at the same concentration, and the observed behaviour was associated with a reduction in inflammatory cytokine produced in the spinal cord.

Details

Title
Andrographolide relieved pathological pain generated by spared nerve injury model in mice
Author
Huang-Chi, Wang 1 ; Hsin-Sheng Tsay 1 ; Hui-Nung Shih 1 ; Yi-An, Chen 1 ; Chang, Kai-Ming 2 ; Agrawal, Dinesh Chandra 1 ; Huang, Siendong 3 ; Yi-Lo, Lin 4 ; Meng-Jen, Lee 1   VIAFID ORCID Logo 

1  Department of Applied Chemistry, Chaoyang University of Technology, Taichung, Taiwan, ROC; 
2  Department of Research, Koo Foundation Sun Yat-Sen Cancer Center, Taipei, Taiwan, ROC; 
3  Department of Applied Mathematics, National Dong Hwa University, Hualien, Taiwan, ROC; 
4  Graduate Institute of Veterinary Pathobiology, National Chung Hsing University, Taichung, Taiwan, ROC 
End page
131
Publication year
2018
Publication date
Dec 2018
Publisher
Taylor & Francis Ltd.
ISSN
13880209
e-ISSN
17445116
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2351041491
Copyright
© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.