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A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences ofLabeo rohita, Cirrhina mrigala andCatla catla have been cloned, characterized and overexpressed inEscherichia coli. These sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.[PUBLICATION ABSTRACT]
Details
MeSH
Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA Primers -- genetics, Escherichia coli -- genetics, Growth Hormone -- chemistry, India, Models, Molecular, Molecular Sequence Data, Pituitary Gland, Protein Conformation, Sequence Analysis, DNA, Carps -- genetics (major), DNA, Complementary -- genetics (major), Growth Hormone -- genetics (major), Nucleic Acid Amplification Techniques -- methods (major)
Identifier / keyword
Title
Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy
Author
Journal of Biosciences; Dordrecht
Volume
27
Issue
3
Pages
261-72
Publication year
2002
Publication date
Jun 2002
Place of publication
Dordrecht
Country of publication
Netherlands
Publication subject
ISSN
02505991
e-ISSN
09737138
Source type
Scholarly Journal
Language of publication
English
Document type
Journal Article
Accession number
12089475
ProQuest document ID
236226408
Document URL
https://www.proquest.com/scholarly-journals/molecular-cloning-growth-hormone-encoding-cdna/docview/236226408/se-2?accountid=208611
Copyright
Indian Academy of Sciences 2002
Last updated
2024-10-04
Database
ProQuest One Academic