Content area

Abstract

A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences ofLabeo rohita, Cirrhina mrigala andCatla catla have been cloned, characterized and overexpressed inEscherichia coli. These sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.[PUBLICATION ABSTRACT]

Details

Title
Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy
Author
Venugopal, T; Mathavan, S; Pandian, T J
Pages
261-72
Publication year
2002
Publication date
Jun 2002
Publisher
Springer Nature B.V.
ISSN
02505991
e-ISSN
09737138
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1007/BF02704915
ProQuest document ID
236226408
Copyright
Indian Academy of Sciences 2002