Abstract

Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have facilitated powerful biological engineering applications, vastly enhancing the scope and efficiencies of genetic editing and transcriptional regulation. In this review, we discuss multiplexed CRISPR technologies and describe methods for the assembly, expression and processing of synthetic guide RNA arrays in vivo. Applications that benefit from multiplexed CRISPR technologies, including cellular recorders, genetic circuits, biosensors, combinatorial genetic perturbations, large-scale genome engineering and the rewiring of metabolic pathways, are highlighted. We also offer a glimpse of emerging challenges and emphasize experimental considerations for future studies.

Multiplexed CRISPR technologies have recently emerged as powerful approaches for genetic editing and transcriptional regulation. Here the authors review this emerging technology and discuss challenges and considerations for future studies.

Details

Title
Multiplexed CRISPR technologies for gene editing and transcriptional regulation
Author
McCarty, Nicholas S 1   VIAFID ORCID Logo  ; Graham, Alicia E 2 ; Studená Lucie 2   VIAFID ORCID Logo  ; Ledesma-Amaro, Rodrigo 2   VIAFID ORCID Logo 

1  California Institute of Technology, Division of Biology and Biological Engineering, Pasadena, USA (GRID:grid.20861.3d) (ISNI:0000000107068890) 
2  Imperial College London, Department of Bioengineering and Imperial College Centre for Synthetic Biology, London, UK (GRID:grid.7445.2) (ISNI:0000 0001 2113 8111) 
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-020-15053-x
ProQuest document ID
2375480233
Copyright
This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.