Abstract

Sex chromosome gene content and expression is unusual. In many organisms, the X and Y chromosomes are inactivated in spermatocytes, possibly as a defense mechanism against insertions into unpaired chromatin. In addition to current sex chromosomes, Drosophila has a small gene-poor X-chromosome relic (4th) that re-acquired autosomal status. Using single-cell RNA-Seq, we demonstrate that the single X and pair of 4th chromosomes are specifically inactivated in primary spermatocytes. In contrast, genes on the single Y chromosome become maximally active in primary spermatocytes. Reduced X steady-state transcript levels are due to failed activation of RNA-Polymerase-II by phosphorylation of Serine 2 and 5.

Footnotes

* https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE125949

* https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE115511

* https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE115478

Details

Title
Dynamic Sex Chromosome Expression in Drosophila Male Germ Cells
Author
Mahadevaraju, Sharvani; Fear, Justin Matthew; Akeju, Miriam; Galletta, Brian J; Pinheiro, Mara Mls; Avelino, Camila C; Cabral-De-Mello, Diogo C; Conlon, Katie; Dell'orso, Stefania; Demere, Zelalem; Kush Mansuria; Mendonca, Carolina A; Palacios-Gimenez, Octavio M; Ross, Eli; Savery, Max; Yu, Kevin; Smith, Harold E; Sartorelli, Vittorio; Rusan, Nasser M; Vibranovski, Maria D; Matunis, Erika; Oliver, Brian
University/institution
Cold Spring Harbor Laboratory Press
Section
New Results
Publication year
2020
Publication date
Mar 23, 2020
Publisher
Cold Spring Harbor Laboratory Press
ISSN
2692-8205
Source type
Working Paper
Language of publication
English
DOI
https://doi.org/10.1101/2020.03.23.000356
ProQuest document ID
2381878698
Copyright
© 2020. This article is published under https://creativecommons.org/publicdomain/zero/1.0/ (“the License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.