Abstract

Extracellular vesicles (EVs) are membranous vesicles that are released by cells. In this study, the role of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery in the biogenesis of yeast EVs was examined. Knockout of components of the ESCRT machinery altered the morphology and size of EVs as well as decreased the abundance of EVs. In contrast, strains with deletions in cell wall biosynthesis genes, produced more EVs than wildtype. Proteomic analysis highlighted the depletion of ESCRT components and enrichment of cell wall remodelling enzymes, glucan synthase subunit Fks1 and chitin synthase Chs3, in yeast EVs. Interestingly, EVs containing Fks1 and Chs3 rescued the yeast cells from antifungal molecules. However, EVs from fks1∆ or chs3∆ or the vps23∆chs3∆ double knockout strain were unable to rescue the yeast cells as compared to vps23∆ EVs. Overall, we have identified a potential role for yeast EVs in cell wall remodelling.

Kening Zhao et al. show that yeast extracellular vesicles are depleted of ESCRT proteins but enriched with Fks1 and Chs3. The toxic effect of antifungal agents can be diminished by exposure to the Fks1- and Chs3- rich extracellular vesicles, suggesting a role for yeast extracellular vesicles in cell wall remodelling.

Details

Title
Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling
Author
Zhao, Kening 1 ; Bleackley, Mark 1   VIAFID ORCID Logo  ; Chisanga, David 2   VIAFID ORCID Logo  ; Lahiru, Gangoda 1 ; Fonseka Pamali 1 ; Liem, Michael 1 ; Kalra Hina 1 ; Al Saffar Haidar 1 ; Keerthikumar Shivakumar 3 ; Ching-Seng, Ang 4 ; Adda, Christopher G 1 ; Jiang Lanzhou 1 ; Yap Kuok 5 ; Poon, Ivan K 1   VIAFID ORCID Logo  ; Lock, Peter 1   VIAFID ORCID Logo  ; Bulone Vincent 5 ; Anderson, Marilyn 1   VIAFID ORCID Logo  ; Mathivanan Suresh 1 

1  La Trobe University, Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, Melbourne, Australia (GRID:grid.1018.8) (ISNI:0000 0001 2342 0938) 
2  La Trobe University, Department of Computer Science and Information Technology, Melbourne, Australia (GRID:grid.1018.8) (ISNI:0000 0001 2342 0938) 
3  La Trobe University, Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, Melbourne, Australia (GRID:grid.1018.8) (ISNI:0000 0001 2342 0938); Peter MacCallum Cancer Centre, Cancer Research Division, Melbourne, Australia (GRID:grid.1055.1) (ISNI:0000000403978434); University of Melbourne, Sir Peter MacCallum Department of Oncology, Melbourne, Australia (GRID:grid.1008.9) (ISNI:0000 0001 2179 088X) 
4  University of Melbourne, Bio21 Institute, Melbourne, Australia (GRID:grid.1008.9) (ISNI:0000 0001 2179 088X) 
5  The University of Adelaide, Waite Campus, ARC Centre of Excellence in Plant Cell Walls and Adelaide Glycomics, Urrbrae, Australia (GRID:grid.1010.0) (ISNI:0000 0004 1936 7304) 
Publication year
2019
Publication date
2019
Publisher
Nature Publishing Group
e-ISSN
23993642
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s42003-019-0538-8
ProQuest document ID
2389679505
Copyright
© The Author(s) 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.