Development and validation of a quick easy cheap effective rugged and safe-based multi-residues analysis method for persimmon, grape and pear using liquid chromatography-tandem mass spectrometry

Abstract

Association of official agricultural chemists official method was adapted and used to develop analytical method for determination of 76 pesticides residues in persimmon, grape, and pear by concurrent use of liquid chromatography-tandem mass spectrometry. Despite few exceptions in particular matrix, two fortified spiking (100 and 400 ppb) in three matrices gave satisfactory results in terms of accuracy, repeatability, precision, and linearity. Method detection limits (MDLs) were determined using five low spiking and eight replicate samples. MDLs were calculated by multiplying the standard deviation with student t-value 2.998 for n-1 (7) degree of freedom at 99% confident level. limit of quantification were obtained by multiplying standard deviation with 10. Experimental results indicate grape was the most problematic matrix among tested fruits and persimmon the least. Etoxazole is the most problematic pesticide and not applicable in this method. Developed method was successfully applied for the determination of residual pesticides in blindincurred samples.