Content area
Abstract
Strain DS4, a soil microorganism producing an oxygen radical-generating antibacterial substance, bruceanol B, was isolated, and cultural conditions for the antibiotic production were optimized. Bruceanol B was separated by ethyl acetate extraction, silica gel and Sephadex LH-20 gel column chromatographies, and high-performance liquid chromatography (HPLC). The highest antibacterial activity was obtained from medium containing 1.5% soluble starch and 2.0% yeast extract, cultured at 30°C for 3.5 days.
Details
Title
Production conditions for bruceanol b, an antibiotic that generates oxygen radical
Author
Lee, Doseung 1 ; Kyung-Hwan, Boo 1 ; Jin-Kyu, Woo 2 ; Jeon Gyeong-Lyong 2 ; Jin-Man, Lee 3 ; Sang-Han, Lee 4 ; Kim, Jae Hoon 5 ; Dong-Sun, Lee 2 ; Riu Key Zung 5
1 Jeju National University, College of Applied Life Science, Jeju, (GRID:grid.411277.6) (ISNI:0000000107255207); Jeju National University, Subtropical Horticulture Research Institute, Jeju, (GRID:grid.411277.6) (ISNI:0000000107255207)
2 Jeju National University, College of Applied Life Science, Jeju, (GRID:grid.411277.6) (ISNI:0000000107255207)
3 Hoseo University, Department of Food & Technology, Asan, (GRID:grid.412238.e) (ISNI:0000000405327053)
4 Kyungpook National University, Department of Food Science and Technology, Daegu, (GRID:grid.258803.4) (ISNI:0000000106611556)
5 Jeju National University, College of Applied Life Science, Jeju, (GRID:grid.411277.6) (ISNI:0000000107255207); Jeju National University, Subtropical Horticulture Research Institute, Jeju, (GRID:grid.411277.6) (ISNI:0000000107255207); Jeju National University, Citrus Genetic Resources Bank, Jeju, (GRID:grid.411277.6) (ISNI:0000000107255207)
1 Jeju National University, College of Applied Life Science, Jeju, (GRID:grid.411277.6) (ISNI:0000000107255207); Jeju National University, Subtropical Horticulture Research Institute, Jeju, (GRID:grid.411277.6) (ISNI:0000000107255207)
2 Jeju National University, College of Applied Life Science, Jeju, (GRID:grid.411277.6) (ISNI:0000000107255207)
3 Hoseo University, Department of Food & Technology, Asan, (GRID:grid.412238.e) (ISNI:0000000405327053)
4 Kyungpook National University, Department of Food Science and Technology, Daegu, (GRID:grid.258803.4) (ISNI:0000000106611556)
5 Jeju National University, College of Applied Life Science, Jeju, (GRID:grid.411277.6) (ISNI:0000000107255207); Jeju National University, Subtropical Horticulture Research Institute, Jeju, (GRID:grid.411277.6) (ISNI:0000000107255207); Jeju National University, Citrus Genetic Resources Bank, Jeju, (GRID:grid.411277.6) (ISNI:0000000107255207)
Pages
128-131
Publication year
2011
Publication date
Feb 2011
ISSN
17382203
e-ISSN
2234344X
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2399178724
Copyright
© Springer-Verlag 2003.