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Abstract
In the process of screening for anti-human acyl-CoA:cholesterol acyltransferase (hACAT) agents from plant resources, we found that the 80% methanolic extract of Rhus chinensis (R. chinensis) exhibited significant antioxidative and hACAT activities in rats. Two compounds were isolated as active principles from the ethyl acetate soluble fraction of the branches of R. chinensis. The structures of compounds 1 and 2 were determined as hydroxydammarenone and semialactone, respectively, by means of MS and NMR spectroscopic analysis. Compound 1 has been isolated from this plant for the first time. Compounds 1 and 2 showed inhibitory activities on both human ACAT1 with IC50 values of 12.4, and 79.1 μM and on human ACAT2 with IC50 values of 30.5 and 76.9 μM, respectively. These results suggested that R. chinensis containing dammarane triterpenes 1 and 2 might be effective in the prevention and treatment of hypercholesterolemia or atherosclerosis via inhibitory effect on hACAT.
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1 National Institute of Horticultural & Herbal Science, Department of Herbal Crop Research, Eumseong, (GRID:grid.420186.9) (ISNI:0000 0004 0636 2782)
2 Korea Research Institute of Bioscience & Biotechnology, Industrial Bio-materials Research Center, Daejeon, (GRID:grid.249967.7) (ISNI:0000000406363099)
3 Kyung Hee University, Graduate School of Biotechnology, Youngin, (GRID:grid.289247.2) (ISNI:0000000121717818)
4 Catholic University of Daegu, School of Medicine, Daegu, (GRID:grid.253755.3) (ISNI:0000000093707312)
5 Kyungpook National University, School of Applied Biosciences, Daegu, (GRID:grid.258803.4) (ISNI:0000000106611556)