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Copyright © 2020 Yang Li et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0/

Abstract

Duck enteritis virus (DEV) can successfully evade the host innate immune responses and establish a lifelong latent infection in the infected host. However, the study about how DEV escapes host innate immunity is still deficient up to now. In this study, for the first time, we identified a viral protein VP16 by which DEV can obviously downregulate the production of IFN-β in duck embryo fibroblast (DEF). Our results showed that ectopic expression of VP16 decreased duck IFN-β (duIFN-β) promoter activation and significantly inhibited the mRNA transcription of IFN-β. Further study showed that VP16 can also obviously inhibit the mRNA transcription of interferon-stimulated genes (ISGs), such as myxovirus resistance protein (Mx) and interferon-induced oligoadenylate synthetase-like (OASL). Furthermore, we found that this anti-interferon activity of VP16 depended on its N-terminus (aa1-200). Coexpression analysis revealed that VP16 selectively blocked duIFN-β promoter activity at the duIRF7 level rather than duIRF1. Based on the results of coimmunoprecipitation analysis (co-IP) and indirect immunofluorescence assay (IFA), VP16 was able to bind to duck IRF7 (duIRF7) directly, but did not interact with duck IRF1 (duIRF1) in vitro.

Details

Title
Duck Enteritis Virus VP16 Antagonizes IFN-β-Mediated Antiviral Innate Immunity
Author
Yang, Li 1 ; Wang, Mingshu 1 ; Cheng, Anchun 1   VIAFID ORCID Logo  ; Jia, Renyong 1 ; Yang, Qiao 1 ; Chen, Shun 1   VIAFID ORCID Logo  ; Zhu, Dekang 2 ; Liu, Mafeng 1   VIAFID ORCID Logo  ; Zhao, Xinxin 1 ; Zhang, Shaqiu 1 ; Huang, Juan 1 ; Ou, Xumin 1 ; Mao, Sai 1 ; Yu, Yanling 1 ; Zhang, Ling 1 ; Liu, Yunya 1 ; Pan, Leichang 3 ; Tian, Bin 3 ; Mujeeb Ur Rehman 3 ; Chen, Xiaoyue 2 

 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang 611130, China 
 Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang 611130, China 
 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang 611130, China 
Editor
Antonio Greco
Publication year
2020
Publication date
2020
Publisher
John Wiley & Sons, Inc.
ISSN
23148861
e-ISSN
23147156
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2406272299
Copyright
Copyright © 2020 Yang Li et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0/