Abstract

Host switch events of influenza A viruses (IAVs) continuously pose a zoonotic threat to humans. In 2013, swine-origin H1N1 IAVs emerged in dogs soon after they were detected in swine in the Guangxi province of China. This host switch was followed by multiple reassortment events between these H1N1 and previously circulating H3N2 canine IAVs (IAVs-C) in dogs. To evaluate the phenotype of these newly identified viruses, we characterized three swine-origin H1N1 IAVs-C and one reassortant H1N1 IAV-C. We found that H1N1 IAVs-C predominantly bound to human-type receptors, efficiently transmitted via direct contact in guinea pigs and replicated in human lung cells. Moreover, the swine-origin H1N1 IAVs-C were lethal in mice and were transmissible by respiratory droplets in guinea pigs. Importantly, sporadic human infections with these viruses have been detected, and preexisting immunity in humans might not be sufficient to prevent infections with these new viruses. Our results show the potential of H1N1 IAVs-C to infect and transmit in humans, suggesting that these viruses should be closely monitored in the future.

Details

Title
Characterization of swine-origin H1N1 canine influenza viruses
Author
Wang, Guojun 1 ; Luiz Gustavo dos Anjos Borges 2 ; Stadlbauer, Daniel 3 ; Ramos, Irene 3 ; Bermúdez González, Maria C 2 ; He, Jianqiao 4 ; Ding, Yangbao 4 ; Zuzhang Wei 4 ; Ouyang, Kang 4 ; Huang, Weijian 4 ; Simon, Viviana 5   VIAFID ORCID Logo  ; Fernandez-Sesma, Ana 6 ; Krammer, Florian 3   VIAFID ORCID Logo  ; Nelson, Martha I 7 ; Chen, Ying 4   VIAFID ORCID Logo  ; García-Sastre, Adolfo 8 

1  The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot, People’s Republic of China; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USA; Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, USA 
2  Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USA; Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, USA 
3  Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USA 
4  College of Animal Science and Technology, Guangxi University, Nanning, People’s Republic of China 
5  Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USA; Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, USA; Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, USA 
6  Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USA; Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, USA 
7  Division of International Epidemiology and Population Studies, Fogarty International Center, National Institutes of Health, Bethesda, USA 
8  Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USA; Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, USA; Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, USA; The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, USA 
Pages
1017-1026
Publication year
2019
Publication date
2019
Publisher
Taylor & Francis Ltd.
e-ISSN
22221751
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1080/22221751.2019.1637284
ProQuest document ID
2427525273
Copyright
© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.